FORMULATION AND PHYSICAL STABILITY TESTING OF CREAM SCRUB PREPARATIONS FROM ETHANOL EXTRACT OF Nelumbo nucifera GAERTN FLOWER AND LEAF

Nelumbo nucifera is an aquatic plant that thrives in muddy and soggy soil, particularly in swampy environments. Nelumbo nucifera is utilized in traditional medicine for various purposes, including the management of diarrhea, tissue inflammation, and homeostasis. The flowers and leaves of Nelumbo nucifera contain many secondary metabolite chemicals, including flavonoids, alkaloids, tannins, and antioxidants. The objective of this study is to ascertain the feasibility of formulating a cream scrub using the ethanol extract of Nelumbo nucifera flowers and leaves. Additionally, the study attempts to discover if concentrations of 3%, 5%, and 7% of this extract can effectively moisturize the skin. This research technique is based on experimentation, involving the creation of simplicial, the production of extracts, the formulation of body scrub preparations using ethanol extracts of Nelumbo nucifera flowers and leaves, and the subsequent evaluation of these body scrub preparations. This study found that the moisture content of Nelumbo nucifera flower ethanol extract cream increased by 41.2% in F1, 46.5% in F2, and 52.9% in F3. The humidity percentage values for Nelumbo nucifera leaf extract cream were obtained as follows: F1 at 38.8%, F2 at 44.4%, and F3 at 47.7%. The ethanol extract derived from the flowers and leaves of Nelumbo nucifera can be prepared and used as a cream scrub. A cream scrub containing Nelumbo nucifera flower and leaf extract at concentrations of 3%, 5%, and 7% can effectively moisturize the skin.


Preparing Nelumbo nucifera Leaf and Flower Extract
The simplicia powder extract of Nelumbo nucifera leaves and flowers was macerated at 1:10, 600 grams of material to 6000 ml of solvent.The extract-making process is: Put 600 grams of simplicial powder in a jar.
Soaked in 4500 cc of 70% ethanol solvent.The container was covered with aluminium foil and left for 5 days, stirring occasionally, then filtered using filter paper to yield a filtrate and residue.
Remaceration with 25 parts of the remaining 1500 ml of 70% ethanol is then performed on the residue.After covering the container with aluminium foil, stir every two hours for two days.
After 2 days, the sample was filtered for residue and filtrate.Mix filtrate 1 and filtrate 2, then evaporate the 70% ethanol liquid extract with a rotary evaporator until thick [14].

Scrub Making Method
Gather tools and materials.

Spreadability Test
The spreadability test ensures the preparation is evenly dispersed on the culture.The spreadability criteria for topicals is 5-7 cm.After placing 1 g in the middle of a round glass covered with another, 50 g was added and left for one minute, and the spreadability diameter was measured.After adding 100 g and waiting one minute, the spreadability diameter was measured.After adding 150 g and waiting one minute, assess spreadability diameter.I finished until enough diameter was produced to see how the load affected preparation spreadability [18].

Irritation Test
The irritation test was carried out by applying the cream scrub preparation behind the ear to 12 volunteers and then observing for 15 minutes the symptoms that occurred.
The reaction observed is the occurrence of irritation on the skin or not [19].

Moisture Test
The effectiveness test was conducted on 15 volunteers and divided into five groups, namely: 1. Group I: 3 volunteers for the blank formula 2. Group II: 3 volunteers for 3% formula The skin condition was checked before and after using the scrub [20].

Stability Test (Cycling Test)
The stability test was conducted using the cycling test method.The cream scrub samples were stored at 4ºC for 24 hours and then transferred to a 40º oven for 24 hours (one cycle).The test was conducted for six cycles, and the physical changes, including organoleptics, homogeneity, and pH, were observed [21].

Hedonic Test
The hedonic test is carried out on aroma, physical appearance, texture, and comfort when using the preparation; the hedonic test is also called the test of a person's preference or preference for a product.

Evaluation of Cream Scrub Preparation Organoleptical Test
Tables 1 and Table 2; organoleptic tests of cream scrub preparations from ethanol extracts of seroma flowers and Nelumbo nucifera leaves were performed on three concentrations with blanks to determine shape, colour, and fragrance.

Test pH
The pH test results for the ethanol extract cream scrub from Nelumbo nucifera flowers and leaves carried out using a pH meter can be seen in Table 5 and Table 6.Nelumbo nucifera flower ethanol extract cream scrub pH measurements showed that the blank, F1, F2, F3, and F4 (positive control) had pH values of 6.7, 6.5, 6.5, and 6.4, respectively.The pH test on Nelumbo nucifera leaf ethanol extract cream scrub showed that the blank has a pH of 6.8, F1 has 6.7, F2 has 6.4,F3 has 6. (2022) research on cream scrubs using red seaweed extract at 5%, 10%, and 15% concentrations, the more extract added to the preparation, the more acidic the pH value and the pH decreases [24].

Scatterability Test
The spreadability test of the cream scrub preparation of ethanol extract of Nelumbo nucifera flowers showed an average of 5.6 cm on F0, 5.5 cm on F1, 5.4 cm on F2, 5.3 cm on F3, and 5.1 cm on F4.Nelumbo nucifera spreadability test findings on F0 average 5.6 cm, F1 is 5.6 cm, F2 is 5.4 cm, F3 is 5.3 cm, and F4 is 5.1 cm.
According to the spreadability test results of the cream scrub of ethanol extract of seroma flower, F0 had 5.6 cm spreadability, F1 5.5 cm, F2 5.4 cm, F3

Irritantation Test
In

Moisture measurement results
showing the percentage increase in skin moisture before week 1 and week 4 after use.
Skin is an organism's outer surface and separates it from the outside world.The skin protects tissue against chemical, physical, and mechanical harm and pathogens [1].Aging skin occurs with age.Many internal and external variables affect aging.Sunlight and other external factors can damage the skin.Skin conditions can be treated.Skincare can be internal or exterior [2tools or machines, while traditional care uses natural ingredients processed manually, such as fruit-based body scrubs, Cosmetics are products used on the skin, hair, nails, and external sexual organs, as well as the teeth and oral mucosa, to clean, perfume, change appearance, enhance body odour, and maintain health.Body scrubs are cosmetics that cleanse the body [3,4].Body scrubs, created from flowers and other plants, help keep skin healthy, smooth, and bright.Scrubs can remove weather-and pollution-induced grime from the skin, making it healthy, clean, and beautiful [5] There are two kinds of scrubs: standard scrubs and modern scrubs.Body scrubs from the past were made with rough ingredients like spices and flour.In the meantime, modern body scrubs are made from scrub granules and lotion, which is generally made from milk.Scrubs come in powder, cream, and whipped forms.It is common for cream scrubs to be formed like a paste or thick dough.They can be used directly on damp skin or skin that has been wet first.
were dissolved in hot water (massII).Add mass I too hot, dried mortar.Constantly crushing slowly added mass II produced a homogenous scrub mass.Add flower extract or Nelumbo nucifera leaves to the scrub at the prescribed concentration, crush again, and add used for the test.In a beaker, 1 gram of the substance is diluted in 100 ml of distilled water.The pH meter shows the number until constant on the left.The pH meter shows the preparation's pH.Three copies were tested for each formula.The cream scrub's pH must match the skin's 4.5-6.5 [17].
for moisture content using a moisture checker on their skin first, then given a cream scrub preparation from ethanol extracts of seroma Nelumbo nucifera and leaves on the marked volunteer's hand skin area.
cream), F2 (light brown), and F3 (brown and cream) smell like green tea.Nelumbo nucifera leaf ethanol extract cream scrub F1, F2, and F3 are light brown, brown, and dark brown, cream-shaped, and smell like green tea.Nelumbo nucifera flower and leaf extracts at different concentrations in each formula and in F3, which has 7% ethanol extract, produce a brown and dark brown color because the higher the concentration, the more intense the color.Research on cream scrubs by researcher on red guava leaf extract at 4%, 6%, and 8% showed a stronger and more intense color at 8% [22].on cream scrubs made from ethanol extracts of Nelumbo nucifera flowers and leaves shows that the preparations are homogeneous by not having poorly mixed parts.This is supported by research on rice husk activated charcoal with concentrations of 8%, 10%, and 12%.Susanna, no parts are unmixed when tested on glass [23].
3, and F4 (positive control) has 6.4.The pH of the preparation was lower than the blank preparation, which is safe for facial skin, as the concentration of ethanol extracts of Nelumbo nucifera flower and leaves increased.The acidity of Nelumbo nucifera flower and leaf extracts may affect this.CO2 entering the container during measurements reacts with water to lower pH.The cream scrub of ethanol extracts of Nelumbo nucifera flowers and leaves has a pH of F2 and F3, similar to the skin.According to Sopianti et al.

Table 1 .
Modified Formula for Nelumbo nucifera Flower Ethanol Extract Cream Scrub

Table 2 .
Modified Formula for Nelumbo nucifera Leaf Ethanol Extract Cream Scrub

Table 5 .
pH test of Nelumbo nucifera Flower Ethanol Extract Cream Scrub the irritant test on volunteers,