BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan
Journal Title: BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan ISSN: P-ISSN 2337-9812 | E-ISSN 2549-1768 DOI Prefix: Prefix 10.22373 by Crossref Editor in Chief: Dr. Samsul Kamal, S.Pd., M.Pd Managing Editor: Mulyadi, M.Pd Publisher: Universitas Islam Negeri Ar-Raniry, Banda Aceh, Indonesia Frequency: Two (2) issues per year (April and September) Citation Analysis: Index Copernicus | Sinta 2 | Google Scholar BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan publishes scientific articles in the field of biology education and technology. Furthermore, this journal bridges the gap between research and practice, providing information, ideas and insights, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. This journal is published by the Department of Biology Education in cooperation with the Center for Research and Community Service (LP2M) Universitas Islam Negeri Ar-Raniry Banda Aceh, Aceh, Indonesia. BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan is indexed in Directory of Open Access Journal (DOAJ) and nationally accredited by DIREKTUR JENDERAL PENGUATAN RISET DAN PENGEMBANGAN KEMENTERIAN RISET, TEKNOLOGI, DAN PENDIDIKAN TINGGI REPUBLIK INDONESIA (SINTA 2). Office Address: Jl. Syeikh Abdul Rauf, Komplek Fakultas Tarbiyah dan Keguruan UIN Ar-Raniry, Darussalam, Kec. Syiah Kuala, Banda Aceh, Aceh, Indonesia, Postal Code: 23111. Email: jurnal.biotik@ar-raniry.ac.id Indexed by:
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Effectiveness of Kalamansi Leaves (Citrofortunella microcarpa) as a Natural Insecticide against Cockroaches (Periplaneta americana)
Synthetic insecticides used to eradicate cockroaches leads to ecosystem damage and pest resistance, so there need environmental friendly natural insecticides. This study aims to evaluate the effectiveness of calamansi leaf extract (Citrofortunella microcarpa) as a natural insecticide against the American cockroach (Periplaneta americana). The leaves of C. microcarpa were extracted using the maceration method with 96% ethanol. The extract was prepared in 5 (five) concentrations: 20%, 40%, 60%, 80%, and 90%. Its effectiveness was tested using a spraying method and observed over 9 (nine) hours on the trial. The data shows decreased in the activity of P. americana and morphological changes that ultimately led to death. Mortality data were analyzed using probit analysis to determine the LC₅₀ value. The phytochemical screening of leaf C. microcarpa extract revealed the presence of tannins, saponins, flavonoids, and alkaloids. The leaf C. microcarpa extract was effective in killing P. americana, with mortality rates of 33%, 83%, 100%, 100%, and 100% at concentrations of 20%, 40%, 60%, 80%, and 90%, respectively, and an LC₅₀ of 23.85%. Leaf C. Microcarpa extract containing tannins, saponins, flavonoids, and alkaloids. The 60% concentration demonstrated the highest effectiveness in increasing the mortality rate of P. americana. Keyword:Citrofortunella microcarpa; Periplaneta americana; Natural Insecticide; Mortality.
Copyright (c) 2026 Rena Lestari, Sutarno, Annisa Puji Astuti, Deni Parlindungan, Dewi Handayani http://creativecommons.org/licenses/by-sa/4.0
Coral Fish Ecological Indices and Relationship with Seawater Physical Parameters at Nasi Island
Coral reef fishes are primary indicators of reef health and providers of essential ecosystem services, including maintaining food web balance, controlling algal growth, and supporting local fisheries. Monitoring the structure of coral reef fish communities is crucial for assessing reef conservation status, particularly in the Nasi Island ecosystem, Aceh, which has experienced coral bleaching and continues to face pressures from fishing activities and tourism. This study assessed species richness, diversity, and similarity of coral reef fishes at three stations (Deudap, Alue Riyeung, Pasie Janeng) during October–November 2024 using Belt Transect and Underwater Visual Census methods. The species richness index of coral reef fishes at Nasi Island was high (7.89–9.65), indicating that the reef ecosystem still provides complex habitats and resources that support diverse taxa. Diversity indices ranged from 2.98–3.49, categorized as moderate to high, reflecting relatively balanced community structures, although dominance by certain species may influence ecosystem functioning. Similarity indices between stations ranged from 52–60%, classified as high, suggesting ecological connectivity and uniform environmental conditions across sites. Integration of these indices confirms that the coral reefs of Nasi Island continue to function as resilient ecosystems, with the capacity to maintain biodiversity and ecological stability despite anthropogenic pressures and the impacts of coral bleaching. These findings provide an important foundation for conservation planning, sustainable resource management, and ecotourism development in the region. Keywords: Coral Reef Fish; Diversity; Similarity Index; Species Richness.
Copyright (c) 2026 M. Ali Sarong, Wiwit Artika, Ismul Huda, Iswadi Iswadi, Nadia Aldyza, Samsul Kamal http://creativecommons.org/licenses/by-sa/4.0
Education on the Protection of Aquatic Biodiversity as an Economic Support for Coastal Communities in the Estuarine Area of ​​Gampong Alue Naga
The estuarine area of Gampong Alue Naga supports high aquatic biodiversity and serves as a primary livelihood source for the local coastal community. However, ecological pressures such as overfishing, mangrove habitat degradation, and water pollution have led to a decline in economically valuable species. The research method used was quantitative descriptive with a participatory approach (participatory action research). Data collection used purposive sampling. The research instruments used were observation guidelines, interviews, and field documentation. Data analysis used quantitative descriptive. The findings indicate the presence of more than 40 aquatic species, including molluscs, crustaceans, and fish, such as Anadara granosa, Scylla serrata, and Penaeus monodon, which hold significant economic value. However, a reduction in size and population was observed in several key species. Community-oriented conservation education integrating local ecological knowledge, sustainable harvesting practices, and value-added seafood processing is recommended to maintain ecological balance and strengthen local economic resilience. Therefore, participatory environmental education plays a strategic role in achieving sustainable ecosystem management and improving coastal community welfare. Keywords: Aquatic biodiversity; Coastal economy; Conservation; Estuary; Environmental education
Copyright (c) 2026 Ibrahim, Aiza Rafsanjani, Jalaluddin, Nurul Akmal, Mohd Isha Awang http://creativecommons.org/licenses/by-sa/4.0
Development of Herbal Booster Product “A5I (Katuk (S. androgynus (L.) Merr.), Moringa (M. oleifera), Fenugreek (T. foenum-graecum L.), Torch Ginger (Etlingera elatior), Cinnamon (Cinnamomum burmannii))” as a Lactagogue Based on Clinical Observational Study
Inadequate exclusive A5I provision is a risk factor for stunting, which remains a major health problem in Indonesia. A polyherbal tea product A5I containing katuk (Sauropus androgynus), moringa (Moringa oleifera), klabet (Trigonella foenum-graecum), torch ginger (Etlingera elatior), and cinnamon (Cinnamomum burmannii) was developed as a natural lactagogum. This study aimed to evaluate the safety of the product through histopathological analysis of vital organs and mammary tissue in female mice after repeated administration. A 28-day subchronic toxicity test was conducted on female mice divided into control and treatment groups. The heart, lungs, kidneys, and mammary organs were harvested for histopathological examination using hematoxylin-eosin staining with a standardized scoring system. Myocardial histopathology showed minimal bleeding without necrosis. The lungs exhibited alveolar septal destruction and mild inflammatory cell infiltration without pulmonary edema, indicating a functional alveocapillary barrier. The kidneys exhibited only mild tubular degeneration without necrosis or inflammatory infiltration. Mammary tissue exhibited extensive ductal dilation and abundant intraluminal secretion distribution, consistent with active lactation. Subchronic administration of A5I polyherbal tea did not cause significant histopathological damage to vital organs, and the mammary findings support its potential lactagogic effect. These findings provide preliminary evidence of the safety of this polyherbal product as a breast milk booster, although further studies with larger sample sizes are needed to confirm its safety and clinical effectiveness. Keyword: Breastfeeding Booster; Histopathology; Lactagogue; Mammary Gland; Mice; Polyherbal
Copyright (c) 2026 Eva Kusumahati, Kintoro Kintoro, Sagiran Sagiran http://creativecommons.org/licenses/by-sa/4.0
LC-MS/MS Based Identification of Antifungal Bioactive Compounds from White Turi (Sesbania grandiflora L.) Stem Bark Extract
White turi (Sesbania grandiflora L.) is a tropical plant belonging to the Fabaceae family that is widely distributed and known for its diverse pharmacological activities. However, the utilization of its stem bark as a source of antifungal agents has been relatively underexplored. This study aimed to identify the phytochemical profile and bioactive compounds of white turi stem bark extract with potential antifungal properties. The sample was extracted using a maceration method with 70% ethanol for 72 hours. Qualitative phytochemical screening was conducted using standard methods, including Mayer, Wagner, Dragendorff, Liebermann–Burchard, and FeCl₃ assays. Further analysis was performed using LC-MS/MS (Agilent 6545 Q-TOF LC/MS System) to identify bioactive compounds. The results revealed the presence of alkaloids, terpenoids, phenolics, tannins, and saponins. LC-MS/MS analysis of the F6 water fraction identified several potential antifungal compounds, including adenine, phenylethyl alcohol, benzenedicarboxylic acid bis (2-ethylhexyl) ester, octadecane, and 2,3-dimethoxybenzaldehyde. These compounds are known to exhibit antifungal mechanisms through disruption of cell membranes, protein denaturation, and inhibition of fungal cellular metabolism. Overall, the findings suggest that white turi stem bark extract has strong potential for development as a natural antifungal agent and as a raw material for plant-based health products. Keyword: Bioactive compounds; natural health products; Sesbania grandiflora L.; LC-MS/MS (Agilent 6545 Q-TOF LC/MS System).
Copyright (c) 2026 A. A. Istri Mirah, A. A. K. Darmadi, N. L. Suriani, I.W. Suanda, I.M. Subrata http://creativecommons.org/licenses/by-sa/4.0
The Implementation Of Problem Based Learning Model Suported By Audio Visual Media To Enhance Student Learning Activities
Low student learning activity in class VII MTsN 2 Banda Aceh on the respiratory system material was marked by minimal student participation in asking questions, discussing, and expressing opinions during the learning process. This condition indicates the need to apply innovative and student-centered learning models and media. This study aims to improve student learning activities through the application of the Problem-Based Learning (PBL) model assisted by audio-visual media. This study uses a Classroom Action Research (CAR) design carried out in two cycles, each covering the stages of planning, implementation of actions, observation, and reflection. The research subjects consisted of 37 seventh-grade students at MTsN 2 Banda Aceh. Data were collected using a learning activity observation sheet covering six indicators, namely visual, oral, listening, writing, mental, and emotional activities. The results showed that the average student learning activity increased from 79% in cycle I, which was categorized as high, to 91% in cycle II, which was categorized as very high. The increase occurred consistently across all learning activity indicators. Thus, the application of the PBL model assisted by audio-visual media proved to be effective in increasing student learning activities in biology learning. Keywords: Learning Activities; Problem-Based Learning; Audio-Visual Media; PTK.
Copyright (c) 2026 Nafisah Hanim, Eriawati, Elita Agustina, Riri Rahmadani Putri, Cut Ratna Dewi http://creativecommons.org/licenses/by-sa/4.0
Development of LKPD Based on Contextual Teaching Learning (CTL) on Ecosystem Material to Train Learning Independence
The aim of this research is to develop contextual teaching materials to increase students' learning independence and optimize the connection between learning materials and real-life contexts. This study uses a research and development method with the ADDIE model which includes the stages of analysis, design, development, implementation, and evaluation. The research subjects consisted of fifth-grade students in the limited and extensive testing stages. Data collection techniques included expert validation sheets, teacher and student response questionnaires, and learning independence observation sheets. Data were analyzed descriptively quantitatively and qualitatively. The results showed that the developed CTL-based LKPD met the criteria of very valid with a validation percentage of media experts of 94% and material experts of 92%. The practicality level in the limited test obtained a percentage of 93%, while in the extensive test it reached an average of 94.5% with a very practical category. The effectiveness of the LKPD is shown by the results of observations of the extensive trial which are in the independent category, with an average score of 15 (Class A) and 14 (Class B), with no students in the less independent category. Based on these results, it can be concluded that the CTL-based LKPD on the ecosystem material developed is declared valid, very practical, and effective in training students' learning independence, so it is suitable for use as teaching materials in science learning in elementary schools. Keyword: Contextual Teaching and Learning; ecosystem; independent learning; research; Student worksheet.
Copyright (c) 2026 Mika Yulianti, Nopriyeni Nopriyeni, Tomi Hidayat http://creativecommons.org/licenses/by-sa/4.0
Management of Lhok Bubon Coastal Ecotourism Integrating Local Wisdom and Coastal Conservation Principles Area
This study aims to analyze the capacity and balance of Lhok Bubon tourism based on sustainable ecotourism and Acehnese local wisdom. The research approach is sequential, starting with quantitative analysis and then deepened qualitatively. Based on an area of ​​120 hectares, the maximum capacity of the area reaches 20,700 visitors per day with two visit cycles. The marine tourism suitability analysis produces a score of 500 in the suitable category. Supporting parameters include depth in the very suitable category, freshwater sources of sandy beaches, and open land cover also have a very suitable category, the basic material of the coral sand substrate has a suitable category. Constraints found in air clarity are less suitable. For snorkeling and diving, most physical parameters of waves, current speed, pH, salinity, and air clarity are in the S1 (Very Suitable) category, except for coral reef diversity (<25%) which is in the N (Not Suitable) category. Overall, Lhok Bubon Beach is worthy of being developed as an ecotourism habitat, but by helping with coral reef restoration, facilities, and strengthening local values, the novelty in this research is the integration of carrying capacity, tourism suitability, and local wisdom of Aceh, in addition to the quantitative-qualitative sequential approach in assessing coastal ecotourism. Keyword: Coastal Conservation; Ecotourism; Lhok Bubon; Local Wisdom
Copyright (c) 2026 izwar_123 Izwar, Riza Ulhaq, Akmal Saputra, Ikhwan Rahmatika Latif, Yusrizal Yusrizal http://creativecommons.org/licenses/by-sa/4.0
Development of a Three-Dimensional DNA Structure Model Based on Integrated Waste Recycling SDG's at SMAN 1 Seulimeum Aceh Besar
Materi struktur DNA sulit dipelajari siswa karena bentuknya tidak dapat dilihat langsung dan komponennya kompleks. Penelitian ini bertujuan merancang model struktur DNA tiga dimensi berbasis daur ulang sampah. Metode yang digunakan adalah R&D (Research and Development) dengan model 4-D yang dimodifikasi (tahap define, design, develop). Subjek penelitian terdiri dari 1 ahli materi, 1 ahli media, dan 25 siswa kelas XII SMAN 1 Seulimeum, Aceh Besar. Teknik pengambilan sampel menggunakan purposive sampling. Data dianalisis secara deskriptif kuantitatif dengan teknik persentase. Hasil penelitian menunjukkan tingkat kelayakan produk sebesar 96,64% (sangat layak), dengan rincian: uji keterbacaan produk 92,42%, validasi ahli media 100%, dan validasi ahli materi 97,5%. Model struktur DNA tiga dimensi berbasis daur ulang sampah terbukti efektif membantu siswa memahami materi DNA. Kata kunci: Daur ulang sampah; DNA; model tiga dimensi.
Copyright (c) 2026 Niea Niea Zahara Phonna, Rahmadina Rahmadina http://creativecommons.org/licenses/by-sa/4.0
Ethnopharmacological Insights into Asteraceae: Traditional Medicine and Therapeutic Potential in the Gayo Highlands, Bener Meriah Regency
The Gayo Highlands communities use Asteraceae species in traditional medicine to treat various ailments. This study aimed to document the ethnopharmacological use of Asteraceae and assess community knowledge regarding their medicinal applications. Data were collected from 48 respondents across eight sub-districts using purposive sampling and structured questionnaires. The study identified 10 Asteraceae species used to treat 10 diseases, including diabetes mellitus (DM), cancer, hypertension, liver disorders, common cold, bloating, cough, fever, toothache, and skin itching. Leaves were the most frequently used plant organ (95.96%), mainly from wild plants growing near home yards or abandoned land. Approximately 81.25% of respondents reported using Asteraceae to manage DM. Standard preparation methods included boiling and pounding, with the leaves of Erigeron sumatrensis Retz being the most widely recognized and utilized for the treatment of DM and cancer. The community’s preference for these plants is attributed to their availability, perceived safety, and efficacy. This study highlights the significant ethnopharmacological potential of Asteraceae in the Gayo Highlands and provides a basis for further pharmacological investigations, particularly on E. sumatrensis. Keyword: Asteraceae; traditional medicine; ethnopharmacology; Gayo Highlands; Erigeron sumatrensis
Copyright (c) 2026 Vivera Ruselli Puspa, Zumaidar, Nurdin, Fitmawati, Nir Fathiya http://creativecommons.org/licenses/by-sa/4.0
Experimental Study on Delayed Cell Death After Thawing and the Effect of Cryoprotective Base Solutions
BACKGROUND: Cryopreservation is a cornerstone technology in regenerative medicine, cell therapy, and biobanking; however, delayed cell death (DCD) occurring 24–48 hours post-thaw remains a critical barrier to maintaining high-quality viable cells—even when immediate post-thaw viability exceeds 95%. OBJECTIVE: To investigate the occurrence of delayed cell death (DCD) in cells after thawing following cryopreservation, and to evaluate the regulatory effect of different cryoprotective base solutions, with a focus on establishing a UW solution-based cryopreservation protocol. MATERIALS AND METHODS: Six cell lines (PESUN, Vero, RD, RK13, VeroE6, L20B) were used to compare two freezing methods (rapid freezing vs. slow freezing) and two cryoprotective base solutions (DMEM vs. UW solution). Cell viability was detected by trypan blue staining, apoptotic cells by Giemsa staining, and cell biological characteristics (proliferation, viral susceptibility) were assessed. RESULTS: 1) DCD peaked at 24 h post-thaw: the mortality rate was 42.2% and apoptotic rate was 28.9% in DMEM-based groups. 2) UW solution (intracellular-type, low Na⁺/high K⁺) significantly reduced DCD: 24 h post-thaw mortality was 21.8% (vs. 43.2% in DMEM) and apoptotic rate was 13.9% (vs. 28.9% in DMEM, both P<0.05). 3) The optimal UW-based protocol was confirmed as: 20% dimethyl sulfoxide (DMSO), 1 min room temperature equilibration, rapid freezing, 37°C water bath thawing, 2.5% glucose in dilution medium, and ≥4-fold dilution. 4) Cells preserved by this protocol maintained pre-cryopreservation properties (proliferation index: 3.27 vs. 3.23; doubling time: 41.8 h vs. 42.2 h; Echovirus B1 log₁₀ TCID₅₀/mL: 7.50 vs. 7.50). CONCLUSION: Post-thaw DCD is closely associated with apoptosis. The UW solution-based cryopreservation protocol effectively reduces DCD while preserving cell function, providing a reliable strategy for clinical cell cryopreservation.
Copyright (c) 2026 YongIl Ho, JongHyong Mun, KumChon Pyo, NamIl Pak, SongGuk Choi, Chol Kang, Chol Li, GyongMan Lim , YongDo Choi http://creativecommons.org/licenses/by-sa/4.0
Effectiveness of Ethanol Extract of Lantana camara as a Biolarvicide Against the Mortality of Aedes aegypti Larvae
Aedes aegypti is the primary vector of dengue hemorrhagic fever (DHF), which remains a major public health concern in Indonesia, including Southeast Sulawesi. Continuous use of chemical larvicides poses risks of resistance development and environmental impacts, thereby necessitating safer alternatives for vector control. This study aimed to evaluate the larvicidal efficacy of ethanol extracts from the leaves and flowers of Lantana camara against A. aegypti larvae. The extracts were prepared through maceration using 96% ethanol, followed by bioassay tests on third- and fourth-instar larvae at concentrations ranging from 10–25 µg/mL. Larval mortality was observed at 24-hour intervals. The results revealed that the leaf extract of L. camaraexhibited higher larvicidal activity than the flower extract, with LC₅₀ and LC₉₀ values of 3.26 µg/mL and 8.31 µg/mL, respectively. Larval mortality increased proportionally with both concentration and exposure time. In conclusion, the leaf extract of L. camara demonstrates potential as an effective and environmentally friendly botanical larvicide for dengue vector control. Keyword: Aedes aegypti; Lantana camara; Larvicide; LC, Mortality
Copyright (c) 2026 Balda Balda, Sry Sukmawaty, Rusni Bagenda http://creativecommons.org/licenses/by-sa/4.0
Development of an Android-Based Electronic Module Using Website 2 APK with Islamic Values on Virus Material for Senior High School Students
Abstract: Innovations in education, including electronic teaching materials, enhance students' motivation and understanding, supported by technological advancements. However, the integration of technology in learning remains suboptimal, as observed in Biology education. The topic of viruses is considered difficult due to its abstract nature and complex scientific terminology, while instruction still relies heavily on textbooks with minimal use of technology. This study aims to develop an Android-based e-module on viruses, integrating Islamic values to facilitate students' understanding. The development follows the ADDIE model and is evaluated by experts before being tested with teachers and students. Validation results indicate a very high feasibility level (98% from subject matter experts, 99% from media experts, and 92% from Islamic values experts). The trial results show high approval ratings from teachers (93.7%) and students (90.1%), demonstrating that the e-module is highly effective in supporting engaging, flexible, and meaningful learning, applicable for both in-person and online education. Keyword: Electronic module 1; Android 2; virus 3; Islamic Values 4.
Copyright (c) 2025 Anggi Aprilia, Agus Sujarwanta, Hening Widowati http://creativecommons.org/licenses/by-sa/4.0
Ethnobotanical Study and Conservation of Medicinal Plants Among Communities in Blang Pegayon, Gayo Lues Regency
Blang Pegayon Subdistrict, Gayo Lues Regency, is one of the regions with high biodiversity and is renowned for its role in traditional medicine. This study aimed to identify the medicinal plant species used by local communities, the plant parts utilized, processing methods, and conservation strategies applied. The research population consisted of residents of Blang Pegayon Subdistrict, Gayo Lues Regency, with purposive sampling involving 48 respondents from six villages based on the presence of traditional medicinal plant use, including both key and non-key informants. Data were collected through semi-structured interviews, observation, plant identification using the Picture This application and the Plants of the World Online (POWO) database, and documentation. The data were analyzed descriptively. The findings revealed 51 medicinal plant species belonging to 29 families. The leaf was the most frequently used plant part (58%), while the most common processing method was pounding (26%). Conservation efforts were carried out through cultivation in home gardens (40%), planting in fields or farms (40%), and the use of wild plants from surrounding environments (20%). The results of this study are expected to provide valuable information for the development of future sustainable research. Keyword: etnobotany; conservation; traditional; medicine plant.
Copyright (c) 2025 Asni Maryam, Djufri, Dewi Andayani, Wardiah, Vivera Ruselli Puspa, Ucha Arief Pratama http://creativecommons.org/licenses/by-sa/4.0
Increasing Student Creativity in Science Learning Through Project Based Learning Models and Animated Videos
proses pelaksanaan pembelajaran IPA di Madrasah lebih banyak terkonsentarsi pada peningkatan aspek pengetahuan sedangkan pengembangan kreativitas siswa lebih sering terabaikan. Dengan demikian sangat pentingmengembalikan pembelajaran IPA sesuai hakekatnya agar pengembnagan kreativitas siswa ikut terkembangkan. Salah satu cara yang dapat dilakukan adalah dengan memilih salah satu model dan media yang cocok untuk mengembangakan kreativitas siswa seperti model PBL dan media video animasi. Tujuan penelitian ini adalah untuk menganalisis aktivitas guru, aktivitas siswa, hasil belajar dan peningkatan kreativitas siswa melalui penerapan model project based learning berbantuan video animasi pada proses pembelajaran IPA kelas IV di MIN 29 Aceh Besar. Metode yang digunakan dalam penelitian ini adalah Penelitian Tindakan Kelas (PTK). Data penelitian dikumpulkan melalui lembar observasi guru, lembar observasi siswa, lembar soal tes dan lembar observasi kreativitas siswa. Hasil penelitian menunjukkan bahwa (1) aktivitas guru pada siklus I dengan rata-rata 84% dan meningkat pada siklus II dengan rata-rata 95%. (2) Aktivitas siswa pada siklus I dengan rata-rata 79% meningkat pada siklus II dengan rata-rata 93%. (3) Hasil belajar siswa pada siklus I 52,17% meningkat pada siklus II 88,46%. (4) Kreativitas siswa pada siklus I dengan rata-rata 56,52% dan meningkat pada siklus II dengan rata-rata 88,46%. Dengan demikian dapat disimpulkan bahwa penerapan model project based learning berbantuan video animasi dapat meningkatkan kreativitas dan hasil belajar siswa kelas IV di MIN 29 Aceh Besar.
Copyright (c) 2025 Wati Oviana, Putri Rahmi, Misbahul Jannah, Eva Nauli Taib, Azza Ariqa http://creativecommons.org/licenses/by-sa/4.0
Controlling Plasmodiophora brassicae Wor. Clubroot with a Dry Formulation of Trichoderma sp. on Broccoli Plants (Brassica oleracea L.)
One of the common diseases in broccoli plants is clubroot caused by soil-borne pathogens (Plasmodiophora brassicae Wor.). One form of prevention of this disease is by applying dry formulation of Trichoderma sp. This study aims to determine the effect of giving dry formulation of Trichoderma sp. in controlling clubroot disease in Broccoli. This study used a Randomized Block Design (RBD) with 4 treatments, namely P0 (0 grams/liter), P1 (5 grams/liter.), P2 (7 grams/liter), and P3 (9 grams/liter) with 6 replications per treatment. The results showed that P3 was the most effective in increasing plant height, leaf blades, leaf length, leaf width, wet weight, and broccoli flower weight. While P2 was the most effective concentration in controlling clubroot disease with the lowest disease severity level of 23.85. Keyword: Dry Formulation; Trichoerma sp.; Broccoli; Mace Root.
Copyright (c) 2026 Yusi Nuraini, Abdul Rahman, Chesara Novatiano http://creativecommons.org/licenses/by-sa/4.0
Integrating Gamified Blended Learning in Biology Education: Enhancing Scientific Literacy and Critical Thinking Skills in Higher Education
Abstract: In the context of Indonesian higher education, conventional learning approaches centered on lecturers are still dominant, thus inhibiting active student involvement and the development of higher-order thinking skills, especially in biology learning that requires conceptual understanding, analytical skills, and scientific application. This study aims to analyze the improvement of students' scientific literacy and critical thinking skills after the implementation of gamified blended learning on the material of Root Anatomy. This study uses Mixed Methods Research with a quasi-experimental design approach. The results of the scientific literacy analysis show a t-value of 9.45, greater than the t table of 1.54 at a significance level of 0.05, and a significance value of 0.00 <0.05. In addition, the results of the t-test on the results of critical thinking skills strengthen these findings with a t-value of 7.48> t table 1.34, which indicates a very significant difference between the average N-gain values ​​of students in the experimental class and the control class. Thus, the alternative hypothesis (Ha) is accepted, which means that the gamified blended learning model is effective in improving students' critical thinking skills and scientific literacy. Keyword: Blended Learning; Gamification; Science Literacy; Critical Thinking Skills.
Copyright (c) 2025 Fatemah Rosma, Mauizah Hasanah, Asy Syifa http://creativecommons.org/licenses/by-sa/4.0
Analysis of the Effects of Tropical Fruit Extracts on the Physicochemical, Tenderness and Organoleptic Characteristics of Buffalo Meat
Buffalo meat is an essential protein source in many regions but faces quality challenges, including oxidation, discoloration, and reduced tenderness. This study investigates the impact of soaking buffalo meat in extracts from melinjau, papaya, and pineapple on its physicochemical properties (color, protein, fat content, and tenderness) and organoleptic characteristics (color, aroma, texture, tenderness, and juiciness). Buffalo meat samples were soaked in fruit extracts for varying durations, followed by analysis of color, protein and fat content, tenderness, and sensory qualities. The results showed significant effects of soaking on color, with melinjau and papaya extracts improving lightness and reducing redness. Soaking with papaya and pineapple extracts enhanced tenderness, while melinjau extract increased juiciness. Protein content decreased slightly, but fat content was significantly reduced, particularly with papaya extract. Sensory evaluation revealed that melinjau extract produced the most favorable tenderness and juiciness, while papaya and pineapple extracts also improved tenderness. These findings suggest that tropical fruit extracts can be used to enhance buffalo meat quality by improving tenderness, reducing fat content, and extending shelf life. This natural preservation method offers a potential alternative to synthetic additives, contributing to better sensory properties and higher-quality buffalo meat products.
Copyright (c) 2025 Faisal Abdurrahman, Othman Othman Omar, Iswadi Iswadi http://creativecommons.org/licenses/by-sa/4.0
Diversity and Distribution of the Macroscopic Species of Fungi in the Forest Park of Pocut Meurah Intan, Aceh, Indonesia
Data on the diversity of macroscopic fungi in Tahura Pocut Meurah Intan is still not widely available, resulting in a gap in basic data that is essential for conservation, area management, and exploration of biological potential. This study aims to identify the diversity of macroscopic fungal species found in PMI National Park and analyze their distribution based on substrate type and habitat conditions. An exploration survey method was used by following existing hiking trails. Fungal samples found in the field were documented, collected, preserved, and then analyzed morphologically. Data analysis was performed using descriptive qualitative methods, presenting species distribution according to habitat conditions, substrate type, and differences between the rainy and dry seasons. Each category was presented as a percentage of the total identified species. 123 macroscopic fungal species were found, with a dominance of the order Agaricales (39%). A total of 28 species have the potential as alternative food sources, while 17 species have the potential as medicine. The highest diversity was found in habitats with heterogeneous vegetation (85.37%), followed by homogeneous forests (21.95%) and agricultural areas (16.26%). Weathered wood was the main substrate where fungi grew (56%), and the number of species was higher in the rainy season than in the dry season. These findings indicate that the PMI Nature Reserve is an important habitat for macroscopic fungi with variations in distribution influenced by differences in substrate and environmental conditions. Keyword: diversity of species, macrofungi, Tahura Pocut Meurah Intan, fungal composition
Copyright (c) 2025 Hendrix Indra Kusuma, Essy Harnelly, Zairin Thomy, Hilmina Itawarnemi http://creativecommons.org/licenses/by-sa/4.0
Exploring the Local Potential of Marine Ecotourism in Sabang Island as a Contextual-Based Resource for Biology Learning
This study aims to analyze and reconstruct the local potential of natural resources in the Iboih ecotourism areas as a source of biology learning. The final target of this study is to be used as references for educators and developers of biology learning in utilize local potential as a context for biology learning. The method used by this study is descriptive research with a qualitative approach. This study was conducted in the Iboih ecotourism areas of Sabang Island, consists of two stages, namely the first exploration of local potential in the ecotourism area, and the second describing local potential as a source of learning based on contextual. Data analysis was carried out using descriptive analysis techniques by using observation sheet that researcher developed. The results of observations and analysis showed that the material that can be used as a source of learning from the Iboih ecotourism areas is related to ecology, with material content about ecosystems, biodiversity, and ecological interactions. Therefore, the study of local potential in the Iboih ecotourism areas is known potential to be used as a source of contextual biology learning, that closely related to eveyday life.
Copyright (c) 2025 Anita Noviyanti, Sri Ismulyati, Dewi Febriyanti, Dian Aswita http://creativecommons.org/licenses/by-sa/4.0
Dry Preparation of Trichoderma Species Promotes Significant Decrease of Fusarium oxysporum in Shallot Cultivation (Allium ascalonicum)
Shallot is a horticultural plant that is susceptible to pathogen attacks. The continuous use of non-biological fungicides has a negative impact on the environment, making it necessary to implement biological control using antagonistic microorganisms. This study aims to evaluate the differences in growth and yield of shallots treated with a dry formulation of Trichoderma sp. to control Fusarium wilt. The study was conducted using a Randomized Block Design (RBD) with four treatments and six replications. The concentrations used were 0 grams/liter, 7 grams/liter, 14 grams/liter, and 21 grams/liter. The data analysis results showed that the dry formulation of Trichoderma sp. at a concentration of 21 grams/liter was the most effective in controlling Fusarium wilt, with the lowest disease severity of 23.16 (F_1,24=47.61, p<0.05). It also significantly increased plant height (F_1,24=6.36, 7.89, 14.24, p<0.05), the number of leaves (F_1,24=15.62, 16.07, 18.29, p<0.05), the number of bulbs (F_1,24=13.57, p<0.05), and biomass weight (F_1,24=57.34, p<0.05). The higher the concentration of the dry formulation of Trichoderma sp. applied, the greater the increase in the vegetative and generative growth of shallot plants.
Copyright (c) 2025 Distira Viro Nika http://creativecommons.org/licenses/by-sa/4.0
Improving Learning Outcomes of High School Students through E-Magazine Learning Media Based on Qur'anic Values on Biodiversity Material
Learning media based on qur'anic values significantly contribute to student outcomes. One reason for low learning outcomes in classes is that teaching materials rooted in Islamic values connect students with moral principles and shape their character, thereby strengthening the integration of science and religion. This research aims to improve student learning outcomes through e-magazine learning media based on qur'anic values. The research design is a quantitative experimental study with a pre-experimental method, specifically the One Group Pretest-Posttest Design. The sample consists of one class, X-3, totaling 30 students, selected using purposive sampling. The instrument used is a test consisting of 20 multiple-choice questions. Data were analyzed using the N-gain formula. The results showed an increase in student learning outcomes, with an average pretest score of 30.19. After instruction with e-magazine learning media based on qur'anic values, the posttest average improved to 80.19. The N-gain value was 0.72, indicating a high level of improvement. It can be concluded that the application of e-magazine learning media based on qur'anic values in biodiversity material significantly enhances student learning outcomes
Analysis of the Implementation of Remedial Biology Learning in the Kurikulum Merdeka
This research was conducted to see the implementation of remedial learning in the independent curriculum at 12 high schools in Central Lampung District. The method used is descriptive qualitative with purposive sampling technique. The results of the research analysis of the feasibility of remedial biology learning in the Independent Curriculum in State High Schools in 12 sub-districts of Central Lampung Regency have carried out and implemented remedial learning in the good category. Analysis of the data obtained in the discussion section can be concluded that from the 4 indicators of teachers at the remedial learning stage, namely identifying remedial learning problems, creating remedial learning plans, implementing remedial learning, and evaluating the process and results of remedial learning, it shows good criteria. Meanwhile, 15 student statements regarding the implementation of Biology remedial learning showed an average score in the good category. Keywords: Biology; Independent Curriculum; Remedial Learning
Effectiveness of Pjbl-Based E-LKPD Implementation in Teaching Plant Tissue Materials
This development research was conducted based on the difficulties faced by teachers. The aim of this research is to produce an e-LKPD that is valid, practical, effective, and appealing for students in the subject of plant tissues. The applied learning model is expected to assist teachers in creating engaging learning media. This research employs the Systematic Literature Review (SLR) method as its research methodology. This involves selecting each relevant journal or prior study and compiling their results as references for the model or idea to be developed. The study involves collecting information and data from reliable sources such as international scientific journals, seminar proceedings, research reports, and reference books. The data in this research consists of a literature review of previous studies sourced from journals published between 2020 and 2024. From several scientific articles that were examined and compared, it can be concluded that several studies using simulation models in their learning processes have been successfully implemented and applied to both students and teachers, at the elementary school and vocational high school levels.
Copyright (c) 2025 Natasya Rahiimah http://creativecommons.org/licenses/by-sa/4.0
Habitat Deterioration of The Golden Horn Bill (Aceros undulatus) in Tahura Pocut Meurah Intan
Pocut Meurah Intan Forest Park, also referred to as Tahura Pocut Meurah Intan, is a protected region located in Aceh Province. The transformation of forested lands into single-crop plantations is leading to significant alterations in the ecological environment. The practice of wood gathering through logging in various sections of the Tahura Pocut Meurah Intan region is interfering with the natural habitats of numerous animal species. This research aimed to assess the habitat loss of the golden hornbill (Aceros undulatus) within Tahura Pocut Meurah Intan. The approach employed for this investigation was an exploratory survey. Data was gathered through a combination of techniques, including the line transect method, concentration count method, and quadrat method. Findings indicated that the habitat degradation affecting the golden hornbill (Aceros undulatus) in Tahura Pocut Meurah Intan was alarmingly severe. In 2021, the extent of habitat damage in the Tahura Pocut Meurah Intan area rose to 2 hectares of primary forest and 21 hectares of tree canopy loss.
Copyright (c) 2025 Samsul Kamal, Mulyadi Mulyadi
Ethnobotany of Anthophyta Varieties as Raw Materials for Candu Oil Production in Tasikmalaya Regency
Pemanfaatan anthophyta sebagai bahan kosmetik ataupun bahan pengobatan yang dilakukan oleh masyarakat tentunya menjadi kajian penting dalam etnobotani. Praktik etnobotani telah turun temurun dilakukan oleh masyarakat Desa Gunungsari dan masyarakat Desa Cikunir. Masyarakat dari dua Desa tersebut menggunakan anthophyta sebagai bahan yang digunakan dalam pembuatan minyak candu. Penelitian ini bertujuan untuk mendeskripsikan minyak candu serta ragam anthophyta yang digunakan dalam proses pembuatannya. Penelitian ini merupakan penelitian kualitatif. Data dikumpulkan dengan cara observasi, wawancara, dan dokumentasi. Sampel diambil menggunakan teknik purposive sampling. Hasil penelitian menunjukan bahwa bagian anthophyta yang banyak digunakan untuk minyak candu yaitu bunga, daun, akar dan rimpang. Terdapat tujuh jenis anthophyta yang diklasifikasikan dalam 2 class dan 5 order. Jenis anthophyta tersebut adalah Cananga odorata, Magnolia x alba, Rosa x damascena, Jasminum sambac, Plantago major, Pandanus amaryllifolius, Curcuma longa. Khasiat yang dirasakan oleh responden sebagai pengguna minyak candu adalah 90%-70% sebagai perawatan kulit kering, 80%-70% untuk pijatan, 60%-50% untuk bekas luka, 55%-35% pengobatan kutu air, 40%-20% untuk iritasi kulit, 30%-25% untuk pembengkakan, 30%-15% untuk stretch mark, dan 20%-15% untuk luka bakar. Kesimpulan yang diambil yakni kandungan yang dimiliki oleh setiap anthophyta bahan minyak candu dapat berpotensi untuk mendukung pengobatan permasalahan kulit.
Copyright (c) 2025 Diana Hernawati, Resti Aulia Dewi, Rinaldi Rizal Putra
The Role of the ASICC-SLICES Model in Promoting Science Literacy and Collaboration Skills: A Pathway to Educational Sustainable Development
The lack of science literacy and collaboration skills can hinder high school students in facing global challenges and supporting environmental sustainability actions. Therefore, developing science literacy and collaboration skills is crucial, particularly in biology education. The ASICC-SLICES model (Adapting, Searching, Interpreting, Creating, and Communicating based on Science Literacy Circles) enhances students' science literacy and collaboration skills. This quasi-experimental study, using a pretest-posttest control group design, aims to evaluate the effect of the ASICC-SLICES model on improving students' science literacy and collaboration skills and its contribution to Educational Sustainable Development. The study population consisted of tenth-grade students from a public high school in Lampung, with a sample of 70 students obtained using the Cluster Random Sampling technique. The research instruments included a science literacy test, questionnaires, observation sheets for collaboration skills, and documentation. Data were analyzed using MANOVA with an α of 0.05. The results showed that the implementation of the ASICC-SLICES model significantly improved students' science literacy and collaboration skills, with n-gain scores for the experimental and control groups (sig) 0.000<0.05 (α). These findings indicate that the ASICC-SLICES model contributes positively to students' awareness and actions in supporting environmental sustainability
research on learning Biology and learning materials
Copyright (c) 2025 Nukhbatul Bidayati Haka, Alfiyani Leoninda, Meita Dwi Solviana, Hardiyansyah Masya, Hardiyansyah Masya, Nur Hidayah
Preparation of Virgin Coconut Oil (VCO) Using the Pineapple Enzymatic Method
Virgin Coconut Oil (VCO) merupakan minyak nabati yang diekstrak dari endosperma kelapa segar dan matang tanpa penyulingan kimia atau panas tinggi. VCO dapat diproduksi dengan beberapa metode, salah satunya adalah metode enzimatik nanas. Hasil produksi VCO enzimatik nanas memiliki keunggulan dibandingkan metode lainnya. Nanas merupakan tanaman yang relatif mudah dibudidayakan di Indonesia, namun pemanfaatan nanas masih belum maksimal, biasanya hanya sebagai olahan makanan atau minuman yang dapat dikonsumsi langsung. Penelitian ini bertujuan untuk mengetahui cara produksi VCO menggunakan metode enzimatik dari buah nanas dan parameter fisik VCO yang dihasilkan. Metode yang digunakan dalam penelitian ini adalah metode campuran. Hasil penelitian ini menghasilkan minyak sebanyak 40 ml dengan kejernihan bening, aroma sedikit menyengat khas kelapa, dan memiliki rasa khas minyak kelapa.
Copyright (c) 2025 Alyyah Fikrotun Nisa, Iffah Nur Fauziyah, Ika Nur Aini, Raden Eliza Wardhani, Dyah Astriani, Sapti Puspitarini
Development of a Virtual Laboratory for Bacterial Culture as a Biology Learning Media
This research aims to develop a virtual laboratory Android application entitled "Bacterial Culture" as an innovative learning media in Microbiology courses. This application simulates the bacterial culture process, including media preparation, sterilization techniques, inoculation, and analysis of results. This research is a Research and Development (R&D) study using the Borg and Gall model which was adapted into six stages. The design validation stage includes validation of media and material experts, namely microbiology lecturers and small group trials involving third semester Biology Education students at IAIN Kudus. The results of expert validation by microbiology course lecturers show that this media obtained a validity score of 91.6% (very valid), and practicality testing by 3rd semester students obtained a score of 61.6% (Special). These findings indicate that the virtual laboratory "Bacterial Culture" is a learning medium that can support students' understanding of bacterial culture practicum before carrying out practicum in the laboratory. This study contributes to the progress of technology-based education which is aligned with the Independent Campus Learning Curriculum (MBKM).
Copyright (c) 2025 Salmanera Senja Lopiani, Muhammad Naufal Muzakki, Wahyunisa Maulia Fahmi, Irma Yuniar Wardhani, Achmad Ali Fikri
The Influence of Herbarium-STEM on Students’ Interest of Becoming the Plant Taxonomists
Taxonomy study in Indonesia is still a concern due to non-innovative learning methods that cause students not interested in learning and conducting research on it. Consequently, it was impacted by the lack of Indonesian Plant Taxonomists. This research aimed to determine the influence of Herbarium-STEM on students' interest in becoming The Plant Taxonomist. It was quasi-experimental research using one group pre-test and post-test design. The sample was 35 college students chosen by using a purposive sampling technique. The data was gathered using pre-test and post-test techniques of questionnaire distribution. Data analysis was carried out with SPSS by 1) descriptive quantitative analysis on interest; 2) analysis of increasing interest using the N-Gain test; 3) hypothesis analysis using t-test and R2. The results were obtained: 1) the average value of the pre-test on interest was 40,14 (low category) while the post-test was 80,71 (high category); 2) the average value of the N-Gain test on increasing interest was 0,70 (high category); 3) the result of t-test was 0,00 < 0,05 and the result of R2 test was 0,614. Thus, Herbarium-STEM influences students' interest in becoming plant taxonomists, with a high level of influence at 61,4%.
qualitative and quantitative research of Biology Education and Technology