Suruhan (Peperomia pellucida L. Kunth) and Senggani (Melastoma malabathricum L.) Leaves as Natural Antioxidant Food Additive

Authors

  • Nunung Kurniasih Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Sunan Gunung Djati Bandung
  • Yusuf Rohmatulloh Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Sunan Gunung Djati Bandung
  • Citra Dewi Pratiwi Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Sunan Gunung Djati Bandung
  • Putri Imarotul Muttaqi Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Sunan Gunung Djati Bandung
  • Mohamad Nurul Azmi B Mohamad Taib School of Chemical Sciences, Universiti Sains Malaysia

DOI:

https://doi.org/10.22373/ekw.v11i2.26503

Keywords:

antioxidant, senggani leaves, suruhan leaves, DPPH, bulk cooking oil

Abstract

Abstract: Food additives are components that affect the nature and/or shape of food. One of them is antioxidants added to food products that contain oil to prevent oxidation that causes damage to food ingredients characterized by a rancid aroma. Suruhan (Peperomia pellucida (L. Kunth)) and Senggani (Melastoma malabathricum L.) leaves are used as natural antioxidants to prevent such damage. This study aims to determine the class of compounds in both plant extracts, their antioxidant activity, and the effect of adding these extracts to bulk cooking oil. The extraction of Suruhan and Senggani leaves is carried out using maceration with ethanol. In phytochemical tests, both Senggani and Suruhan leaf extracts showed positive results in containing flavonoids, tannins, phenolics, and saponins. An antioxidant activity test using the DPPH method (2,2-diphenyl-1-picrylhydrazyl) measured absorption at a maximum wavelength of 518 nm and compared it with antioxidant synthesis TBHQ (tert-butyl hydroquinone). The results showed that Suruhan and Senggani leaves had moderate antioxidant activity with IC50 values of 131.5575 and 119.0362 ppm, respectively. The two extracts with the highest inhibitory value of 38.3657% were then applied to bulk cooking oil, which was heated at 60°C and 180°C and compared to the addition of TBHQ to bulk cooking oil. The results showed that the natural antioxidants of Suruhan and Senggani leaf extracts could inhibit damage to bulk cooking oil during the heating process by reducing the peroxide number and free fatty acid levels in bulk cooking oil.

Abstrak: Bahan tambahan pangan merupakan komponen yang ditambahkan untuk mempengaruhi sifat dan atau bentuk suatu bahan pangan. Salah satunya adalah antioksidan yang ditambahkan pada produk pangan yang mengandung minyak dengan tujuan mencegah oksidasi penyebab rusaknya bahan pangan yang ditandai dengan aroma tengik. Daun suruhan (Peperomia pellucida (L.) Kunth) dan senggani (Melastoma malabathricum L.) digunakan sebagai antioksidan alami dalam mencegah kerusakan tersebut. Penelitian ini bertujuan untuk mengetahui golongan senyawa yang terkandung dalam kedua ekstrak tanaman, aktivitas antioksidan, dan pengaruh penambahan ekstrak tersebut pada minyak goreng curah. Ekstraksi daun suruhan dan senggani dilakukan dengan metode maserasi menggunakan etanol. Pada uji fitokimia, baik ekstrak daun senggani maupun suruhan menunjukkan hasil positif mengandung flavonoid, tanin, fenolik, dan saponin. Dan hanya daun suruhan yang mengandung steroid. Uji aktivitas antioksidan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazil) diukur serapan pada panjang gelombang maksimum 518 nm dan dibandingkan dengan antioksidan sintesis TBHQ (tert-butyl hydroquinon). Hasil penelitian menunjukkan bahwa daun suruhan dan senggani memiliki aktivitas antioksidan yang sedang dengan nilai IC50 berturut-turut sebesar 131,5575 dan 119,0362 ppm. Kedua ekstrak dengan nilai inhibisi tertinggi yaitu 38,3657% selanjutnya diaplikasikan pada minyak goreng curah yang dilakukan pemanasan pada suhu 60°C dan 180°C serta dibandingkan dengan adanya penambahan TBHQ pada minyak goreng curah. Hasil penelitian menunjukkan bahwa antioksidan alami ekstrak daun suruhan dan senggani dapat menghambat kerusakan pada minyak goreng curah selama proses pemanasan berlangsung dengan menurunkan bilangan peroksida dan kadar asam lemak bebas pada minyak goreng curah.

References

Ayucitra, A., Indraswati, N., Yulianus, VM., Kurniawan D; Francisco, G., Yudha, A., 2013, “Potensi Senyawa Fenolik Bahan Alam Sebagai Antioksidan Alami Minyak Goreng Nabati,” Widya Tek., vol. 10, no. 1, pp. 1–10, [Online]. Available: http://jurnal.wima.ac.id/index.php/teknik/article/view/155

Bobiński, R and Bobińska, J., 2002. “Fatty acids of human milk – a review,” Int. J. Vitam. Nutr. Res., vol. 92, no. 3–4, pp. 280–291, Jul., doi: 10.1024/0300-9831/a000651.

Boateng, L., Ansong, R., Owusu, W., and Steiner-Asiedu, M., 2016, “Coconut oil and palm oil’s role in nutrition, health and national development: A review,” Ghana Med. J., vol. 50, no. 3, pp. 189–196, Oct. doi: 10.4314/gmj.v50i3.11.

Brinkmann, B., 2000., “Quality criteria of industrial frying oils and fats,” Eur. J. Lipid Sci. Technol., vol. 102, no. 8–9, pp. 539–541, Sep, doi: 10.1002/1438-9312(200009)102:8/9<539::AID-EJLT539>3.0.CO;2-B.

Erickson, M. D., Yevtushenko, D. P., and Lu, Z.-X., 2023, “Oxidation and Thermal Degradation of Oil during Frying: A Review of Natural Antioxidant Use,” Food Rev. Int., vol. 39, no. 7, pp. 4665–4696, Aug. doi: 10.1080/87559129.2022.2039689.

Elizabeth, L., Machado, P., Zinöcker, M., Baker, P. and Lawrence, M. 1955, “Ultra-Processed Foods and Health Outcomes: A Narrative Review,” Nutrients, vol. 12, no. 7, p. Jun. 2020, doi: 10.3390/nu12071955.

Feiner, G., 2016. Salami : Practical Science and Processing Technology,. London : Academic Press, doi: 10.1016/C2015-0-06154-8.

Higea, J. F., Azizah, Z., Rasyid, R. and Kartina, D. 2016. “Pengaruh pengulangan dan lama penyimpanan terhadap ketengikan minyak kelapa dengan metode asam thiobarbiturat (tba),” vol. 8, no. 2, pp. 189–200

Jideani, A. I. O., Silungwe, H., Takalani, T., Omolola, A. O., Udeh, H. O. and Anyasi, T. A. 2021, “Antioxidant-rich natural fruit and vegetable products and human health,” Int. J. Food Prop., vol. 24, no. 1, pp. 41–67, Jan. doi: 10.1080/10942912.2020.1866597.

Jouki, M. and Khazaei, N. 2022, “Effects of active batter coatings enriched by quince seed gum and carvacrol microcapsules on oil uptake and quality loss of nugget during frying,” J. Food Sci. Technol., vol. 59, no. 3, pp. 1104–1113, Mar., doi: 10.1007/s13197-021-05114-4.

Kornienko J. S., Smirnova, I.S., Pugovkina, N.A., Ivanova, J.S., Shilina, M. A., Grinchuk, T.M., shatrova, A.N., Aksenov, N.D., Zenin, V.V., Nikolsky N.N., dan Lyublinskaya O.G., 2019, “High doses of synthetic antioxidants induce premature senescence in cultivated mesenchymal stem cells,” Sci. Rep., vol. 9, no. 1, p. 1296, Feb. doi: 10.1038/s41598-018-37972-y.

Latino, M. E., De Devitiis, B., Corallo, A., Viscecchia, R. and Bimbo, F., 2022, “Consumer Acceptance and Preference for Olive Oil Attributes—A Review,” Foods, vol. 11, no. 23, p. 3805, Nov. doi: 10.3390/foods11233805.

Lempang, I.R., Fatimawali, Palealu, N. C., 2016. “Uji kualitas minyak goreng curah dan minyak goreng kemasan Di Manado,” Pharmacon, Vol 5 No 4, doi.org/10.35799/pha.5.2016.13987

Lichtenstein A. H,. Appel, L.J., Vadiveloo, M., Hu, F.B., Kris-Etherton, P.M, Rebholz, C.M., Sacks, F.M, Thorndike, A.N., Horn, L.V., Wylie-Rosett, J., 2021, “Dietary Guidance to Improve Cardiovascular Health: A Scientific Statement From the American Heart Association,” Circulation, vol. 144, no. 23, Dec. doi: 10.1161/CIR.0000000000001031.

Liu, R. and Mabury, S. A. 2020, “Synthetic Phenolic Antioxidants: A Review of Environmental Occurrence, Fate, Human Exposure, and Toxicity,” Environ. Sci. Technol., vol. 54, no. 19, pp. 11706–11719, Oct. doi: 10.1021/acs.est.0c05077.

Ma, J.K. Li, K., Li , X, Elbadry, S., Raslan , A.A., Li, Y., Mulla, Z.S., Tahoun, A.B.M.B., El-Ghareeb, W.R., Chen Huang, X., 2021, “Levels of polycyclic aromatic hydrocarbons in edible and fried vegetable oil: a health risk assessment study,” Environ. Sci. Pollut. Res., vol. 28, no. 42, pp. 59784–59791, Nov. doi: 10.1007/s11356-021-14755-z.

Nam, K., Lim, H. and Ahn, B. 2020, “Information Values on the Consumer’s Valuation of non-GM Material: The Case of Cooking Oil in Korea,” Sustainability, vol. 12, no. 19, p. 7876, Sep. doi: 10.3390/su12197876.

Popkin B. M., Barquera, S., Corvalan, C., Hofman, K.J., Monteiro, C., Ng, S.W., Swart, E.C., Taillie, L.S., 2021, “Towards unified and impactful policies to reduce ultra-processed food consumption and promote healthier eating,” Lancet Diabetes Endocrinol., vol. 9, no. 7, pp. 462–470, Jul. doi: 10.1016/S2213-8587(21)00078-4.

Rahaman, M. M., Hossain, R., Herrera‐Bravo, J., Islam, M.T., Atolani, O., Adeyemi, O.S., Owolodun, O.A., Kambizi, L., Daştan, S.D., Calina, D., Sharifi‐Rad, J., 2023, “Natural antioxidants from some fruits, seeds, foods, natural products, and associated health benefits: An update,” Food Sci. Nutr., vol. 11, no. 4, pp. 1657–1670, Apr. doi: 10.1002/fsn3.3217.

Ribeiro, J. S., Santos, M.J.M.C., Silva, L.K.R., Pereira, L.C.L., Santos, I.A., Lannes, S.C.S., da Silva, MV., 2019, “Natural antioxidants used in meat products: A brief review,” Meat Sci., vol. 148, pp. 181–188, Feb. doi: 10.1016/j.meatsci.2018.10.016.

Sacks, F. M., 2020, “Coconut Oil and Heart Health,” Circulation, vol. 141, no. 10, pp. 815–817, Mar. doi: 10.1161/CIRCULATIONAHA.119.044687.

Touvier, M., 2020, “Ultra-processed/Ultra-formulated foods: association with chronic disease risk,” Eur. J. Public Health, vol. 30, no. Supplement_5, Sep. doi: 10.1093/eurpub/ckaa165.1406.

Wang, W., Xiong, P., Zhang, H., Zhu, Q., Liao, C. and Jiang, G., 2021, “Analysis, occurrence, toxicity and environmental health risks of synthetic phenolic antioxidants: A review,” Environ. Res., vol. 201, p. 111531, Oct., doi: 10.1016/j.envres.2021.111531.

Wang, H., Liu, X., Tu, M., Xu, X., Yang, S. and Chen, D. 2022, “Current sample preparation methods and analytical techniques for the determination of synthetic antioxidants in edible oils,” J. Sep. Sci., vol. 45, no. 20, pp. 3874–3886, Oct. doi: 10.1002/jssc.202200358.

Wang, Y., Wu, X., McClements, D. J., Chen, L., Miao, M. and Jin, Z., 2021, “Effect of New Frying Technology on Starchy Food Quality,” Foods, vol. 10, no. 8, p. 1852, Aug. doi: 10.3390/foods10081852.

Wiege, B., Fehling, E., Matthäus, B. and Schmidt, M., 2020, “Changes in Physical and Chemical Properties of Thermally and Oxidatively Degraded Sunflower Oil and Palm Fat,” Foods, vol. 9, no. 9, p. 1273, Sep., doi: 10.3390/foods9091273.

Xu, X., Liu, A., Hu, S. Ares, I., Martínez-Larrañaga, M., Wang, X., Martínez, M., Anadón,A., Martínez, M.A., 2021, “Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action,” Food Chem., vol. 353, p. 129488, Aug. doi: 10.1016/j.foodchem.2021.129488.

Yeo, J., Lee, J., Yoon, S. and Kim, W. J., 2020, “Tannic acid-based nanogel as an efficient anti-inflammatory agent,” Biomater. Sci., vol. 8, no. 4, pp. 1148–1159, doi: 10.1039/C9BM01384A.

Zafar, F., Asif, H.M., Shaheen, G., Ghauri, A.O., Rajpoot , S.R., Tasleem, M.W., Shamim, T., Hadi, F., Noor, R., Ali, T., Gulzar, M.N., Nazar, H., 2023, “A comprehensive review on medicinal plants possessing antioxidant potential,” Clin. Exp. Pharmacol. Physiol., vol. 50, no. 3, pp. 205–217, Mar. doi: 10.1111/1440-1681.13743.

Zhang, Y., Zhang, T., Liang, Y., Jiang, L. and Sui, X., 2021, “Dietary Bioactive Lipids: A Review on Absorption, Metabolism, and Health Properties,” J. Agric. Food Chem., vol. 69, no. 32, pp. 8929–8943, Aug. doi: 10.1021/acs.jafc.1c01369.

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31-12-2025

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