EFFECT OF GIVING MATOA FRUIT PEEL EXTRACT ON LEVELS OF SUPEROXIDE DISMUTASE (SOD), MALONDIALDEHYDE (MDA) AND HISTOLOGICAL FEATURES OF THE LUNGS OF RATS GIVEN CIGARETTE SMOKE EXPOSURE

Authors

  • Levinna Levinna Universitas Prima Indonesia, Medan, Indonesia
  • Tan Suyono Universitas Prima Indonesia, Medan, Indonesia
  • Linda Chiuman Universitas Prima Indonesia, Medan, Indonesia

DOI:

https://doi.org/10.22373/lj.v11i2.19113

Keywords:

Cigarette smoke, Histopathology of lungs, Matoa peels extract, MDA, SOD

Abstract

The matoa plant (Pometia pinnata) is known as a medicinal plant that has high antioxidant activity. Cigarette smoke produces free radicals and causes oxidative stress, a condition where the number of free radicals in the body exceeds the capacity to neutralize them. The aim of this study was to evaluate the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and histopathological features of rat’s lung exposed to cigarette smoke and matoa peels extract. The research method uses laboratory experimental studies using rats as research subjects. The design used was Randomized Posttest Only with Control Group Design. 24 male white rats of the wistar strain were divided into 6 groups: normal, negative control, positive control, and group I, II, III were given matoa peels extract 3, 6, and 12 mg/kgBW respectively and exposure to cigarette smoke for 14 days. After 14 days, MDA, SOD and histopathological picture of the rats' lungs will be compared based on the group. Data analysis was carried out using a one-way ANOVA test. The results showed that matoa peels extract could reduce MDA levels and increase SOD activity in rats exposed to cigarette smoke (p value <0.05). Group that is given matoa peels extract 12 mg/kgBW can reduce lung tissue damage.

References

Adyttia, A., Untari, E. K., & Wahdaningsih, S. (2014). Efek ekstrak etanol daun Premna cordifolia terhadap malondialdehida tikus yang dipapar asap rokok. Pharmaceutical Sciences and Research, 1(2), 5.

Andriani, M., Nahrowi, N., Jayanegara, A., Mutia, R., & Syahniar, T. M. (2020). Antioxidant Quality of Phytochemical Compounds and Chemical Characteristics of Dried Matoa (Pometia Pinnata) Peels. Jurnal Veteriner, 21(4), 604-610.

Agustiarini, V., & Wijaya, D. P. (2022). Uji aktivitas antioksidan ekstrak etanol-air (1: 1) bunga rosella (Hibiscus sabdariffa L.) dengan metode DPPH (1, 1-difenil-2-pikrilhidrazil). Jurnal Penelitian Sains, 24(1), 29-32.

Dilema Industri rokok Indonesia (2018) Direktorat P2PTM. Available at: http://p2ptm.kemkes.go.id/kegiatan-p2ptm/dilema-industri-rokok-indonesia (Accessed: November 4, 2022).

Fauziah, A., Sudirga, S. K., & Parwanayoni, N. M. S. (2021). Uji antioksidan ekstrak daun tanaman leunca (Solanum nigrum L.). Metamorfosa: Journal of Biological Sciences, 8(1), 28.Hajar, S. et al. (2021) “Potensi Ekstrak Buah Matoa (Pometia Pinnata) sebagai Sumber Antioksidan: Literature Review,” Jurnal Farmasi Sains dan Praktis (JFSP), 7(1), pp. 59–66.

Hardi, R. (2014). Pengaruh Terapi Rebusan Akar Gantung Pohon Beringin (Ficus benjamina L.) Terhadap Kadar Malondialdehida (MDA) dan Profil Pita Protein Serum Tikus (Rattus norvegicus) Hasil Paparan Asap Rokok (Doctoral dissertation, Universitas Brawijaya).

Hasan, H., Thomas, N. A., Hiola, F., Ramadhani, F. N., & Ibrahim, A. S. (2022). Skrining Fitokimia dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) Dengan Metode 1, 1-Diphenyl-2 picrylhidrazyl (DPPH). Indonesian Journal of Pharmaceutical Education, 2(1), 67-73.

Herdiani, N., & Putri, E. B. P. (2018). Gambaran histopatologi paru tikus wistar setelah diberi paparan asap rokok. Medical and Health Science Journal, 2(2).

Irawan, C., Hanafi, L. S., Rochaeni, H., & Lestari, P. S. (2017). Comparison of total phenolic content in seed, flesh fruit and peel of Pometia pinnata from Indonesia. Seed, 177(118.80), 122-18.

Kandungan Dalam sebatang rokok (no date) Direktorat P2PTM. Available at: http://p2ptm.kemkes.go.id/infografhic/kandungan-dalam-sebatang-rokok (Accessed: November 4, 2022).

Matoa - Pertanian (2021) Balai Pengkajian Teknologi Pertanian Papua. Kementrian Pertanian. Available at: http://papua.litbang.pertanian.go.id/index.php/sdg/537-plasmanutfah-3 (Accessed: November 4, 2022).

Mengenal Buah Matoa Yang Manis Dan Eksotis (2021) Dinas Pertanian Pangan Kabupaten Demak. Available at: https://dinpertanpangan.demakkab.go.id/?p=3564 (Accessed: November 4, 2022).

Nasution, A. S., Wirjatmadi, B., & Adriani, M. (2016). Efek Preventif Pemberian Ekstrak Kulit Buah Naga Berdaging Super Merah (Hylocereus Costaricensis) terhadap Malondialdehid Tikus Wistar yang Dipapar Asap Rokok. Jurnal Kedokteran Brawijaya, 29(1), 21-24.

Parwata, O.A. (2015) Antioksidan. Universitas Udayana.

Rahmah, W., Hamzah, H., Hajar, S., Ressandy, S. S., & Putri, E. M. (2021). Potential of Matoa Fruit Extract (Pometia pinnata) As Antioxidant Source. Jurnal Farmasi Sains dan Praktis, 7(1), 59-66.

Rochmah, W. W. (2017). Pengaruh Pemberian Sari Buah Kurma (Phoenix dactylifera) Terhadap Kadar Malondialdehid (MDA) Mencit Balb/c yang Dipapar Asap Rokok.

Triana, N., Ilyas, S., & Hutahaean, S. (2013). Gambaran histologis pulmo mencit jantan (Mus musculus L.) setelah dipapari asap rokok elektrik. Saintia Biologi, 1(2), 1-7.

Tristantini, D., Ismawati, A., Pradana, B. T., & Jonathan, J. G. (2016). Pengujian aktivitas antioksidan menggunakan metode DPPH pada daun tanjung (Mimusops elengi L). In Seminar Nasional Teknik Kimia" Kejuangan" (p. 1).

Wulandari, E. (2016). Efek Ekstrak Kulit Buah Rambutan terhadap Kadar MDA dan SOD Tikus yang Dipapar Asap Rokok. skripsi). Semarang: Universitas Negeri Semarang.

Downloads

Additional Files

Published

2023-12-12

Issue

Vol. 11 No. 2 (2023)

Section

Artikel

License

Proposed Policy for Journals That Offer Open Access Authors who publish with Lantanida Journal agree to the following terms: a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal. c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).