septiani septiani


This research aims to produce large amounts of lipase and have high activity so that it can be utilized in the food industry. Method of Research used a thermostable enzyme derived from compost AL96 microorganisms. Qualitative tests are carried out using Thermus media. Extracellular lipase enzyme expressed by AL96 culture at 700C for 17 hours was isolated as much as 1000 mL and the crude extract obtained was deposited by fractionation of ammonium sulfate. After obtaining a fraction of 0-30%; 30 -50% fraction; 50-70% fraction; and 7-90% fraction was then tested for lipase enzyme activity using spectrophotometric techniques and testing of protein content determined by the Bradford method. Results Of research obtaining partial purification using ammonium sulfate into crude enzyme extract from AL96 isolate with a fraction of 50-70% resulted in the highest specific activity of protein 0.018 U / mg. Further analysis of lipase in the 30-50% fraction has the optimum temperature at 65 0C and the fraction of 50-70% has the optimum temperature at 75 0C. Characterization of the optimum pH for 50-70% fraction and 30-50% fraction showed that both fractions had optimum pH 10.


thermostable lipase; isolate AL96; ammonium sulphate fractions; characterization of lipase; food industry

Full Text:



Bradford, M., M. (1976). A Rapid and Sensitive Method for The Quantitation Microgram Quantities of Protein Utilizing The Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248 – 254.

Cutler, P. (2004). Protein Purification Protocol Second Edition Method in Molecular Biology. Human Press, 244, 24 – 26.

Fardiaz, S. (1992). Analisis Mikrobiologi Pangan. Jakarta: Raja Grafindo Persada.

Jegannathan RK, Nielsen HP. (2013). Environmental Assessment of Enzyme Use in Industrial Production. Journal of Cleaner Production. 42, 22.

Lee, D., Koh, Y., Kim, K., Choi, H., Kim, D., Suhartono, M. T., dan Pyun, Y. (1999). Isolation and Characterization of a Thermophilic Lipase from Bacillus thermoleovorans ID-1, FEMS Microbiology Letters, 179, 393 – 400.

Nurhasanah. (2015). Kloning, Karakterisasi Dan Ekpresi Gen Pengkode Lipase Termostabil dari Kompos Melalui Pendekatan Metagenom, Disertasi Program Doktor, Institut Teknologi Bandung.

Ogunbanwo S. T and Adewara, A. O. (2013). Effects of Processing Variables on The Production Of ‘Burukutu’, A Nigerian Fermented Beverage. Nature and Science Journal, 1-11.

Sari, D. K. (2010). Modifikasi Lipase Isolat Lokal pada Sintesis Biodiesel, Tesis Program Magister, Institut Teknologi Bandung.

Sambrook, J., Fritsch, E. F., Maniatis, T. (1989). Molecular cloning : a Laboratory Manual 3rd edition, USA : Cold Spring Harbor Laboratory Press, 18.47.

Scopes, R. K., (1994). Protein Purification Principles and Practice. New York: Spiringer-Verley Inc.

Sharma, S., dan Kanwar, S. S. (2014). Organic Solvent Tolerant Lipases and Applications. Scientific World Journal, 1 – 16.

Suhartono, M.T. (1989). Enzim dan Bioteknologi. Bogor: PAU Bioteknologi IPB.

Zeikus, G.J., dan Vieille, C. (2001). Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms For Thermostability, 65, 1 – 43.

DOI: http://dx.doi.org/10.22373/lj.v7i1.4696

Article Metrics

Abstract view : 0 times
PDF - 0 times


  • There are currently no refbacks.

Copyright (c) 2019 septiani septiani

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.