PENGARUH EKSTRAK DAUN LIDAH BUAYA (Aloe vera L.) TERHADAP PERTUMBUHAN JAMUR Candida albicans SECARA IN VITRO
DOI:
https://doi.org/10.22373/biotik.v5i1.2977Keywords:
Aloe vera L., Candida albicans, Inhibit ZoneAbstract
The purpose of this study was to determine the ability of aloe vera leaf extract (Aloe vera L.) in inhibiting the growth of Candida albicans fungi through in vitro. Aloe vera (Aloe vera L.) is a plant that can overcome digestive problems caused by fungi. One of the pathogenic fungi causing gastrointestinal infections is C. albicans. This research was conducted in Microbiology Laboratory of FMIPAUnsyiah which lasted from April to May 2010. A Completely Randomized Design (RAL) consisted of 5 treatments and 5 replications is used in this study. The treatment was arranged on P0 = 20 μl distilled water, P1 = aloe vera leaf extract concentration 100%, P2 = aloe leaf extract concentration of 50% and P3 = aloe leaf extract concentration of 25%. Aloe vera leaf extract (Aloe vera L.) was given to each treatment at 20 μl. The parameter observed was the diameter of the inhibit zone formed. The data were analyzed using Variant Analysis (ANOVA), then continued with the Smallest Significant Difference (BNT) test. The results of this study indicated that the presence of aloe vera leaf extract (Aloe vera L.) could inhibit the growth of C. albicans. The higher the concentration of aloe vera leaf extract (Aloe vera L.), the higher the ability of it to inhibit C. albicans. The diameter of the inhibitory zone formed will enlarge along with the increasing of the concentration of aloe vera leaf extract (Aloe vera L.). The extract concentrations of 100%, 50% and 25% had their resistance capability of 0.50 mg, 0.24 mg and 0.20 mg of nistatin antibiotics.References
Bunchanan, R. E. dan Gibbons, N. E. 1974. Bergey’s Manual of Determinative Bacteriology. USA: Waverly Press, Inc.,Baltimore.
Chairani. 2004. Pengaruh Ekstrak Daun Widuri Terhadap Pertumbuhan Staphylococcus aureus Secara In vitro. Skripsi. Banda Aceh: Universitas Syiah Kuala.
Gholib, D. 2009. Daya Hambat Ekstrak Kencur (Kaempferia galanga L.) Terhadap Trichophyton mentagrophytes Dan Cryptococcus neoformans Jamur Penyebab Penyakit Kurap Pada Kulit Dan Penyakit Paru. Balai Besar Penelitian Veteriner. Bul. Littro, 20: 59 – 67.
Jawetz, E., J.L. Melnick and E.A. Adelberg. 2001. Mikrobiologi Kedokteran Edisi-22. Diterjemahkan dari Medical Microbiology Twenty Second Ed oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Jakarta: Salemba Medika.
Fardiaz, S. 1989. Petunjuk Laboratorium Analisis Mikrobiologi Pangan. Depdikbud Direktorat Jenderal Pendidikan Tinggi Pusat Antar Universitas Pangan dan Gizi. IPB.
Jawetz, E., J.L. Melnick and E.A. Adelberg. 1986. Mikrobiologi untuk Profesi Kesehatan. Diterjemahkan dari Review of Medical Microbiology oleh Gerard Bonang. Edisi 16. Jakarta: EGC.
Jawetz, E., J.L. Melnick and E.A. Adelberg. 1996. Mikrobiologi Kedokteran. EGC, Jakarta. Diterjemahkan dari Medical Microbiology Twenty Second Ed oleh Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Jakarta: EGC.
Ariawan, E. 2013. Pengaruh Gel Aloe vera ( Aloe Berbandesis Miller ) Terhadap Uji Daya Hambat Jamur Candida albicans Pada Penyakit Kandidiasis. Makassar: Fakultas Kedokteran Gigi niversitas Hasanuddin.
Rofiatiningrum, A., dkk. 2015. Penggunaan Gel Lidah Buaya (Aloe vera L.) Sebagai Antijamur Pada Dendeng Daging Sapi Giling. Bandung: Fakultas Peternakan Universitas Padjadjaran.
Ariyanti, N.K., dkk. 2012. Daya Hambat Ekstrak Kulit Daun Lidah Buaya (Aloe vera Barbadensis Miller) Terhadap Pertumbuhan Bakteri Staphylococcus aureus atcc 25923 dan Escherichia coli atcc 25922. Bali: Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana.
Ariane, I. 2009. Pengaruh Ekstrak Lidah Buaya (Aloe vera) Terhadap Pertumbuhan Pseudomonas aeruginosa Pada Pasien Osteomielitis Bangsal Cempaka Rumah Sakit Ortopedi Prof. Dr. Ir. Soeharso Surakarta In Vitro. Surakarta: Fakultas Kedokteran Universitas Sebelas Maret Surakarta.
Rahayu, I.D. 2006. Aloe barbadensis Miller Dan Aloe chinensis Baker Sebagai Antibiotik Dalam Pengobatan Etnoveteriner Unggas Secara In Vitro. Malang: Jurusan Peternakan, Fakultas Peternakan Perikanan, Universitas Muhamadiyah.
Armiati, I.G.K., 2015. Ekstrak Etanol Kulit Daun Lidah Buaya (Aloe vera barbadensis Miller) konsentrasi 100% dapat menurunkan akumulasi plak gigi dan jumlah koloni bakteri Streptococcus mutans. Denpasar: Program Pascasarjana Universitas Udayana.
Downloads
Published
Issue
Section
License
Authors who publish with BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.