Larvicidal Activity of Streptomyces sp. Liquid Cultures Against Aedes aegypti Larvae

Ni Wayan Desi Bintari, Putu Ayu Parwati


Abstract: The tropics have significant future challenges in controlling the vectors of dengue hemorrhagic fever. The occurrence of resistance to chemical control encourages the development of strategies based on biological control. This study aimed to test the larvicidal activity of Streptomyces sp. liquid culture toward A. aegypti larvae. The selection of Streptomyces as a bio-larvicide was carried out by a chitinase test. Chitinase-producing bacteria were cultivated in biomass culture. The study was conducted using a completely randomized design. The results of this study can be isolated 4 Streptomyces isolates from muddy soil. Among the isolates, Streptomyces sp.4 showed chitinolytic activity on in vitro tests; therefore, it was used for larvicidal activity. Based on the Duncan test result, Streptomyces sp.4 culture showed a significant effect on larval mortality compared to the negative control (p<0.05). The highest rate of larval mortality was found in the A6B1 treatment (34.35%). The results of the Probit test showed that the LD50 value of the Streptomyces sp.4 culture was 24.6±5.4 mL. Based on the in vivo test, showed that Streptomyces sp.4 liquid culture affected the mortality rate of A. aegypti larvae and was significantly different from the negative control (p<0.05). Streptomyces sp.4 is known to have potential benefits as a biological larvicidal agent.

Abstrak: Daerah tropis memiliki tantangan besar kedepannya dalam pengendalian vektor demam berdarah dengue (DBD). Adanya kejadian resistensi pengendalian dengan zat kimiawi mendorong strategi pengembangan berbasis pengendalian biologis. Penelitian ini bertujuan untuk melakukan pengujian aktivitas kultur Streptomyces sp. sebagai larvasida A. aegypti. Seleksi Streptomyces sebagai biolarvasida dilakukan dengan uji aktivitas kitinase. Bakteri penghasil kitinase dilakukan kultivasi kultur biomassa untuk pengujian in vivo. Rancangan uji menggunakan Rancangan Acak Lengkap. Hasil penelitian dapat diisolasi 4 isolat Streptomyces yang diisolasi dari tanah berlumpur. Diantara keempat isolat, satu isolat yaitu Streptomyces sp.4 menunjukkan aktivitas kitinolitik sehingga digunakan untuk uji aktivitas larvasida secara in vivo. Hasil uji larvasida menunjukkan perlakuan kultur Streptomyces sp.4 berpengaruh nyata terhadap persentase kematian larva dibandingkan kontrol negatif (p<0.05) berdasarkan hasil uji Duncan. Persentase kematian tertinggi didapatkan pada perlakuan A6B1 yaitu sebesar 34.35%. Hasil uji Probit menunjukkan nilai LD50 dari kultur Streptomyces sp.4 adalah 24.6 ± 5.4 mL. Berdasarkan hasil uji in vivo diketahui bahwa perlakuan kultur cair Streptomyces sp.4 berpengaruh terhadap tingkat kematian larva A. aegypti dan berbeda nyata dengan kontrol negative (p<0.05). Streptomyces sp. 4 diketahui memiliki potensi sebagai salah satu agen larvasida biologis.


chitinolytic bacteria; dengue hemorrhagic fever; integrated vector management

Full Text:



Aggarwal, C., Paul, S., Tripathi, V., Paul, B., & Khan, M. A. (2015). Chitinolytic activity in Serratia marcescens (strain SEN) and potency against different larval instars of Spodoptera litura with effect of sublethal doses on insect development. BioControl, 60(5), 631–640.

Al-Qwabah, A. A., Al-Limoun, M. O., Al-Mustafa, A. H., & Al-Zereini, W. A. (2018). Bacillus atrophaeus A7 crude chitinase: characterization and potential role against Drosophila melanogaster larvae. Jordan Journal of Biological Sciences, 11(4), 451–459.

Atikasari, E., & Sulistyorini, L. (2018). Pengendalian vektor nyamuk Aedes aegypti di Rumah Sakit Kota Surabaya. The Indonesian Journal Public Health, 13(1), 71–82.

Basik, A. A., Juboi, H., Shamsul, S. S. G., Sanglier, J. J., & Yeo, T. C. (2020). Actinomycetes isolated from wetland and hill paddy during the warm and cool seasons in Sarawak, east Malaysia. Journal of Microbiology, Biotechnology and Food Sciences, 9(4), 774–780.

Benelli, G., Jeffries, C. L., & Walker, T. (2016). Biological control of mosquito vectors: Past, present, and future. Insects, 7(4), 1–18.

Bintari, N. W. D., Kawuri, R., & Dalem, A. A. G. R. (2015). Streptomyces sp. as a biocontrol of vibriosis on larvae of Macrobrachium rosenbergii (de Man) Prawns. Journal of Biological and Chemical Research, 34(1), 238–248.

Boukaew, S., Petlamul, W., Suyotha, W., & Prasertsan, P. (2016). Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease. Biotechnologia, 97(4), 271–284.

Braks, M., Giglio, G., Tomassone, L., Sprong, H., & Leslie, T. E. (2019). Making vector-borne disease surveillance work: new opportunities from the SDG perspectives. In Frontiers in Veterinary Science (Vol. 6, Issue 232, pp. 1–9). Frontiers Media S.A.

Chater, K. F. (2016). Recent advances in understanding Streptomyces F1000Research, 5, 1–16.

Fatmawati, U., Meryandini, A., Nawangsih, A. A., & Wahyudi, A. T. (2019). Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas, 20(10), 2970–2977.

Harapan, H., Michie, A., Mudatsir, M., Sasmono, R. T., & Imrie, A. (2019). Epidemiology of dengue hemorrhagic fever in Indonesia: Analysis of five decades data from the National Disease Surveillance. BMC Research Notes, 12(1), 2–6.

Harir, M., Bendif, H., Bellahcene, M., Frotas, Z., & Pogni, R. (2018). Streptomyces secondary metabolites. In Basic Biology and Applications of Actinobacteria. IntechOpen.

Haryanto, B. (2018). Indonesia Dengue Fever: Status, Vulnerability, and Challenges. In Current Topics in Tropical Emerging Diseases and Travel Medicine. IntechOpen.

Jabeen, F., Hussain, A., Manzoor, M., Younis, T., Rasul, A., & Qazi, J. I. (2018). Potential of bacterial chitinolytic, Stenotrophomonas maltophilia, in biological control of termites. Egyptian Journal of Biological Pest Control, 28(1), 1–10.

Jha, S., Modi, H. A., & Jha, C. K. (2016). Characterization of extracellular chitinase produced from Streptomyces rubiginosus isolated from rhizosphere of Gossypium sp. Cogent Food and Agriculture, 2(1), 1–12.

Kurniawati, S., Mutaqin, K. H., & Giyanto. (2015). Eksplorasi dan uji senyawa bioaktif bakteri agensia hayati untuk pengendalian penyakit kresek pada padi. J. HPT Tropika, 15(2), 170–179.

Lacombe-Harvey, M. È., Brzezinski, R., & Beaulieu, C. (2018). Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution. In Applied Microbiology and Biotechnology (Vol. 102, Issue 17, pp. 7219–7230). Springer Verlag.

Law, J. W. F., Ser, H. L., Khan, T. M., Chuah, L. H., Pusparajah, P., Chan, K. G., Goh, B. H., & Lee, L. H. (2017). The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Frontiers in Microbiology, 8(3), 1–10.

Poorna, C. A., & Pradeep, N. S. (2016). Identification of novel chitinolytic Streptomyces. spp from a sacred grove and it’s in vitro antagonistic activity analysis. International Journal of Current Microbiology and Applied Sciences, 5(8), 916–928.

Salas-Ovilla, R., Gálvez-López, D., Vázquez-Ovando, A., Salvador-Figueroa, M., & Rosas-Quijano, R. (2019). Isolation and identification of marine strains of Stenotrophomona maltophilia with high chitinolytic activity. PeerJ, 2019(1), 1–12.

Santhi, R. (2016). Isolation of chitinase producing Streptomyces albus FS12, production and optimization of extracellular chitinase. Int. J. Adv. Res. Biol. Sci, 3(4), 229–237.

Shrivastava, P., Kumar, R., & Yandigeri, M. S. (2017). In vitro biocontrol activity of halotolerant Streptomyces aureofaciens K20: A potent antagonist against Macrophomina phaseolina (Tassi) Goid. Saudi Journal of Biological Sciences, 24(1), 192–199.

Soeka, Y. S., & Triana, E. (2016). Pemanfaatan Limbah Kulit Udang untuk Menghasilkan Enzim Kitinase dari Streptomyces macrosporeus InaCC A454 U. J.Kim.Terap.Indones, 18(1), 91–101.

Sukalkar, S. R., Kadam, T. A., & Bhosale, H. J. (2018). Optimation of chitinase production from Streptomyces macrosporeus M1. Life Science Informatics Publications, 4(1), 106–114.

Veliz, E. A., Martínez-Hidalgo, P., & Hirsch, A. M. (2017). Chitinase-producing bacteria and their role in biocontrol. In AIMS Microbiology (Vol. 3, Issue 3, pp. 689–705). AIMS Press.

Vinodhkumar, T., Subhapriya, M., Gamanathan, G., Suresh, J.I., Kalpana. (2015). Screening of bioactive potential for larvicidal activity of marine actinomycetes. Int.J.Curr.Microbiol.App.Sci, 4(2): 972-980.,%20et%20al.pdf.

Widiastuti, D., & Marbawati, D. (2016). Efek larvasida bakteri kitinolitik dari limbah kulit udang terhadap larva Aedes aegypti. Aspirator, 8(1), 47–54.

Yotopranoto, S., Kurnijasanti, R., Rohmah, E.A. (2017). Isolation of Streptomyces sp. from lapindo mud soil, Sidoarjo, East Java Province, Indonesia as a larvicide candidate against Aedes aegypti. Folia Medica Indonesiana, 53(2), 118-123.

Zhou, G., Lo, E., Githeko, A. K., Afrane, Y. A., & Yan, G. (2020). Long-lasting microbial larvicides for controlling insecticide-resistant and outdoor transmitting vectors: a cost-effective supplement for malaria interventions. Infectious Diseases of Poverty, 9(1), 1–8.



  • There are currently no refbacks.

Copyright (c) 2022 Ni Wayan Desi Bintari, Putu Ayu Parwati

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

P-ISSN : 2460-8912
E-ISSN : 2460-8920


Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.

View full page view stats report click here

Flag Counter