Analisis Ekstrak Etanol Tangkai Daun Buasbuas (Premna pubescens) Menggunakan Gas Chromatography Mass Spectrophotometer (GCMS)

Diky Setya Diningrat, Martina Restuati, Kusdianti Kusdianti, Ayu Nirmala Sari, Erly Marwani


Buasbuas (Premna pubescens) is a plant that is traditionally known to have medicinal properties. This study aims to determine the content of bioactive compounds contained in the petiole by Gas Chromatography Mass Spectrophotometer (GCMS) method. Preparation of ethanol extract of petiole using maceration method with 96% ethanol solvent. The study used gas chromatographic tools and mass spectra which were evaluated using MASSLAB program. The data obtained from the GCMS machine is then analyzed using the NCBI database pubchem software ( The results of this study indicate that the content of bioactive compounds on the petioles of buasbuas more than 50 libraries contains about 150 species of compounds with a range of RT and% area respectively 4.684 to 28.155 and 0.16 to 15.56%. The content of bioactive compounds shown this data indicates that very large potential of buasbuas plants to be explored and exploitation as a nutritious plant. The results of this study can be used as the foundation in the development program of the potential utilization of bioassemblance of buasbuas plants. In further research it is necessary to analyze the other parts of the plant and make comparisons to complete the available databases.


Senyawa bioaktif; Buasbuas; GCMS; Pubchem; ekstrak etanol

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Diningrat, D.S., Restuati, M., Pratiwi, N. and Hanim, N., 2016. Bioactive Compounds In Buasbuas (Premna pubescens. Blume) Shoots With Proteomic Approaches Using Gc-Ms.

Hanim, N. 2016. Pengaruh Ekstrak Etanol Daun Buasbuas (Premna pubescens Blume) Terhadap Penurunan Kadar Gula Darah dan Gambaran Histologi Pankreas Tikus Putih Jantan (Rattus norvegicus) yang Diinduksi Aloksan., Skripsi, FMIPA, UNIMED, Medan.

Hardiyanti, Y. 2016. Efek Toksik Formaldehid Terhadap Organ Reproduksi Tikus Betina (Rattus norvegicus. L) yang Diberikan Ekstrak Etanol Daun Buasbuas (Premna pubescens Blume)., Skripsi, FMIPA, UNIMED, Medan.

Harley, R. M., Atkins, S., Budantsev, A. L., Cantino, P. D., Conn, B. J., Grayer, R., Harley, M. M., de Kok, R. P. J., Krestovskaja, T., Morales, R., Paton, A. J., Ryding, O. & Upson, T. 2004. Labiatae. In: K.Kubitzki & J. W. Kadereit (eds), The Families and Genera of Vascular Plants, Springer- Verlag, Berlin 7: 167- 275

Hidayat, U. 2015. Pengaruh Ekstrak Daun Buasbuas (Premna pubescens Blume) Terhadap Pertumbuhan Bakteri Bacillus cereus Secara In Vitro., Skripsi, FMIPA, UNIMED, Medan.

Leeratiwong, C., Chantaranothai, P dan Paton A. 2016. Taxonomic Notes On The Genus Premna L. (Lamiaceae) In Thailand, Thai Forest Bull., Bot 44: 122-124

Marbun, E.M.A dan M. Restuati. (2015), Pengaruh Ektrak Etanol Daun BuasBuas (Premna pubescens Blume) Sebagai Antiinflamasi Pada Edema Kaki Tikus Putih (Rattus novergicus), Jurnal Biosains 1: 2443-1230

Priyadi, H., Takao, G., Rahmawati, I., Supriyanto, B., Ikbal Nursal, W. and Rahman, I., 2010. Five hundred plant species in Gunung Halimun Salak National Park, West Java: a checklist including Sundanese names, distribution and us. CIFOR, Bogor, Indonesia

Restuati, M., Ilyas, Syafruddin., Hutahaean, Salomo. and Sipahutar, Herbert. .2014. Study of the extract activities of Buas buas leaves (Premna pubescens) as immunostimulant on rats (Rattus novegicus), American Journal of BioScience 2: 244-250

Restuati, M., U. Hidayat, A. S. S. Pulungan, N.Pratiwi. and D. S. Diningrat. 2016. Antibacterial Activity of Buasbuas (Premna pubescens Blume) Leaf Extracts Against Bacillus cereus And Escherichia coli, Journal of Plant Sciences 1816-4951

Restuati, M., Diningrat, D.S. and Hanim, N., 2016. Effect of Ethanol Extract Leaf Buasbuas (Premna pubescens. Blume) To Decrease Blood Sugar Levels in Rats Male (Rattus norvegicus) The Induced Alloxan.,%20Diky%20Setya%20Diningrat,%20Nisfa%20Hanim.pdf

Roessner, U., Wagner, C., Kopka J Trethwey R.N and Wilmitzer, L. 2000. Simultaneous Analysis of Metabolites in Potato Tuber by Gas Chromathography-Mass Spectrometry, the plant journal 23: 131-142

Senja, R.Y., Issusilaningtyas, E., Akhmad Kharis Nugroho, A.K., and Setyowati, E.P., (2014), Perbandingan Metode Ekstraksi dan Variasi Pelarut Terhadap Rendemen dan Aktivitas Antioksidan Ekstrak Kubis Ungu (Brassica oleracea L. var. capitata f. rubra), Traditional Medicine Journal 19: 43-48

Sermakkani, M and V. Thangapandian, 2012. GC-MS Analysis Of Cassia Italica Leaf Methanol Extract. Asian J Pharm Clin Res, Vol 5, Issue 2, 2012, 90-94

Simanjuntak, K. (2012), Pengaruh Pemberian Ekstrak Daun Buasbuas (Premna pubescens Blume) Terhadap Imunitas Humoral Tikus Putih (Rattus norvegicus. L)., Skripsi, FMIPA, Unimed, Medan.



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