The UV-Vis Study On Anthocyanin Pigments Activities Extracted From Gayo Arabika Coffee Husks

Hasby Hasby, Nurhafidhah Nurhafidhah, Said Ali Akbar


The anthocyanin activity of Gayo Arabica coffee husk in variations in acid solution, temperature, and pH has been assessed through UV-Vis studies. It is important to optimize these factors to increase the long-term stability of anthocyanins for use in various fields. Gayo Arabica coffee husks was extracted through maceration method with methanol. This process obtained a coffee husks extract of 1.176 mg/μL. The UV-Vis spectrum of the extract displayed the major absorption peak at λmax= 529 nm in hydrochloric acid (HCl), this assigned by the electronic transition from flavylium as the particular characteristic of anthocyanin compounds. The anthocyanin from the extract was stable at temperatures of 35oC and 50oC, to have a major absorption peak at λmax= 529 nm. Therefore, it could probably be said that the lower the temperature, the higher the absorbance value of anthocyanin. Then, husks extract was getting red at pH 1 and 3; almost colorless at pH 5, 8 and 10; and yellow at pH 12. The more acidic conditions causes more anthocyanin pigments to be observed in the form of flavylium or oxonium cations color. Finally, Gayo Arabica coffee husks extract can absorb both ultraviolet (UV) and visible light (visible).


anthocyanin; coffee husks; Uv-vis study

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Aal, E. S. S. M. A., Hucl, P., & Rabalski, I. 2018. Compositional and Antioxidant Properties of Anthocyanin-rich Products Prepared from Purple Wheat. Food Chem, 254, 13-19.

Abdel-Aty, A. M., Salama, W. H., Fahmy, A. S., & Mohamed, S. A. 2018. Impact of Germination on Antioxidant Capacity of Garden Cress: Newcalculation for Determination of Total Antioxidant Activity. Scientia Horticulturae, 246 (27), 155-160.

Abugri, D.A., Apea, O.B. and Pritchett, G.. 2012. Investigation of a Simple and Cheap Source of a Natural Indicator for Acid-Base Titration: Effects of System Conditions on Natural Indicators. Green and Sustainable Chemistry, 2, 117-122.

Ariadi, H., R. 2015. Ekstraksi Senyawa Antioksidan Kulit Buah Kopi: Kajian Jenis Kopi dan Lama Maserasi. Berkala Ilmiah Pertanian. 1 (1).

Bueno, J.M., Plaza, P.S., Escudero, F.R., Jimenez, A.M., Fett, R. & Asuero, A.G.. 2012. Analysis and Antioxidant Capacity of Anthocyanin Pigments. Part II: Chemical Structure, Color, and Intake of Anthocyanins. Critical Review in Analytical Chemistry, 42 (2): 126-151.

Chen, G. L. Fan, M. X., Wu, J. L., Li, N., & Guo, M. Q. 2018. Antioxidant and Anti Inflammatory Properties of Flavonoids from Lotus Plumule. Food Chemistry, 277 (30), 706-712.

Faria, A.F., Marcella, C.M. and Adriana, Z.M. 2011. Identification of Bioactive Compounds from Jambolão (Syzygium cumini) and Antioxidant Capacity Evaluation in Different pH Conditions. Food Chemistry, 126, 1571-1578.

Fatonah, N. 2016. Uji Stabilitas Zat Warna Ekstrak Buah Sengani. Jurnal Sains dan Matematika. 5 (1): 29-35

Hsiao, Y. H., & Hsieh, J. F. 2018. The Conversion & Deglycosylation of Isoflavones and Anthocyanins in Black Soymilk Process. Food Chem, 261, 8-14.

Ibrahim, U.K., Muhammad, I.I. & Salleh, R.M. 2011. The Effect of pH on Color Behavior of Brassica oleracea Anthocyanin. Journal of Applied Sciences, 11(13), 2406-2410.

Moloney, M. 2018. Red Cabbage Anthocyanins: The Influence of D-glucose Acylation by Hydroxycinnamic Acids on Their Structural Transformations in Acidic to Mildly Alkaline Conditions and on the Resulting Color. Dyes and Pigments, 158, 342-352.

Moulana, R., dkk. 2012. Efektifitas Penggunaan Jenis Pelarut dan Asam dalam Proses Ektraksi Pigmen Antosianin Kelopak Bunga Rosella (Hibiscus sabdariffa L). Jurnal Teknologi dan Industri Pertanian Indonesia. 4, (3): 20-25.

Murthy, P.S. & Naidu, M.M. 2012. Sustainable Management of Coffee Industry By-Products and Value Addition—a review. Resources, Conservation and Recycling, 66, 45-58.

Murthy, P.S., Manjunatha, M.R. Sulochannama, G. & Naidu, M.M.. 2012. Extraction, characterization and Bioactivity of Coffee Anthocyanins. European Journal of Biological Sciences, 4 (1), 13-19.

Nayanathara, A.R., Anu, M., Aalolam, K.P., & Reshma, J.K. 2016. Evaluation of total Phenol, Flavonoid and Anthocyanin Content in Different Varieties of Eggplant. Emer Life Sci Res, 2, (2), 63-65.

Novelina, Nazir, N., & Adrian, M., R. 2016. The Improvement Lycopene Availability and Antioxidant Activities of Tomato (Lycopersicum esculentum, Mill) Jelly Drink. Agriculture and Agricultural Science Procedia, 9, 328-334.

Oliveira, L.S., & Adriana, S.F. 2015. An Overview of the Potential Uses for Coffee Skins. Elsevier, 283-291,

Ryu, D., & Koh, E. 2018. Stability of Anthocyanins in Bokbunja (Rubus occidentalis L.) Under in Vitro Gastrointestinal Digestion. Food Chem, 267, 157-162.

Sampebarra, A., L. 2018. Karakteristik Zat Warna Antosianin dari Biji Kakao Non Fermentasi sebagai Sumber Zat Warna Alam. Jurnal Indutri Hasil Perkebunan. 13, (1): 63-70.

Sekretariat Ditjen Perkebunana Kementrian Pertanian Indonesia. 2017. Statistik Perkebunan Indonesia Komiditas Kopi 2015-2017.

Sui, X. 2014. Combined Effect of pH and High Temperature on The Stability and Antioxidant Capacity of Two Anthocyanins in Aqueous Solution. Food Chemistry., 163, 163-170.

Trinafianita, E., & Widyaningsih, D., T. 2018. Kajian Perlakuan Awal Bahan dan Proporsi Penyeduhan Kopi Bubuk: Air pada Proses Pembuatan Kopi dari Kulit Buah Kopi Arabika (Coffea arabica L.) Lereng Bromo. Jurnal Pangan dan Agroindustri. 6, (4): 59-69

Uranga, J., Etxabide, A., Guerrero, P., & Caba, K. D. L. 2018. Development of Active Fish Gelatin Films with Anthocyanins by Compression Molding. Food Hydrocoll, 84, 313-320.

Yufniati, Z., A. 2015. Pengembangan Model Bioindustri Pertanian Berbasis Kopi Arabika di Dataran Tinggi Gayo Provinsi Aceh. Aceh: Balai Pengkajian Teknologi Pertanian Aceh, Kementerian Pertanian



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Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.

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