Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Robby Gus Mahardika, Occa Roanisca, Fajar Indah Puspita Sari


Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.

Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.


Inhibition of α-glucosidase; acetone extract; Tristaniopsis merguensis Griff.; Brine Shrimp Lethality Test; BSLT

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Ahmed, F., Jnns, C., & Nv, T. (2009). An In Vitro Study on the inhibitory Activities of Eugenia jambolana Seeds Against Carbohydrate Hydrolyzing Enzymes. J .Young Pharm., 1(4), 317–321.

Albuntana, A., Yasman, & Wardhana, W. (2011). Toxicity test of extracts of the four sea cucumber (Family holothuriidae) from East Penjaliran island, Seribu Islands, Jakarta based on the brine shrimp lethality test (BSLT). Jurnal Ilmu Dan Teknologi Kelautan Tropis, 3(1), 65–72.

Dewi, R. T., Tachibana, S., & Darmawan, A. (2013). Effect on a-glucosidase inhibition and antioxidant activities of butyrolactone derivatives from Aspergillus terreus MC751. Medicinal Chemistry Research, 23(1), 454–460.

Dhone, B. S., Cunha, T., & Darmakusuma, D. (2018). Uji toksisitas ekstrak karang lunak sarcophyton sp asal timor terhadap larva artemia salina leach. Chem. Notes, 1(1), 32–40.

Enggiwanto, S., Istiqomah, F., Daniati, K., Roanisca, O., & Mahardika, R. G. (2018). Ekstraksi daun pelawan ( Tristaniopsis merguensis ) sebagai antioksidan menggunakan Microwave Assisted Extraction ( MAE ). Indonesian Journal of Pure and Applied Chemistry, 1(2), 50–55.

Feng, J. Y.-W. (2011). Bio-assay guided isolation and identification alpha-glucosidase inhibitors from the leaves of Aqualaria sinensis. Phytochemistry, 72, 242-247.

Goa, H. H. (2008). a-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L. Food Chemistry, 106, 1195-1201.

Kahn, C. R. (1996). Genetics of non-insulin-dependent (type-II) diabetes mellitus. Annu. Rev. Med., 104, 509-31.

Khuankaew, S. S. (2014). Ethnobotanical study of medicinal plants used by Tai Yai in Northern Thailand. Journal of Ethnopharmacology, 151, 829-938.

Kumar, S. N. (2011). Alpha-glukosidase inhibitors from plants: A natural approach to treat diabetes. Pharmacognosy Reviews, 5, 19-29.

Mahardika, R. G., & Roanisca, O. (2018). Antioxidant activity and phytochemical of extract ethyl acetate pucuk idat (Cratoxylum glaucum ). Indo. J. Chem. Res, 5(2), 481–486.

Mahardika, R. G., & Roanisca, O. (2019). Microwave-Assisted Extraction of polyphenol content from Leaves of Tristaniopsis merguensis. ASEAN Journal of Chemical Engineering, 19(2), 110–119.

Maobe, M. A. G., Nyarango, R. M., & Box, P. O. (2013). Fourier Transformer Infra-Red Spectrophotometer Analysis of Urtica dioica Medicinal Herb Used for the Treatment of Diabetes, Malaria and Pneumonia in Kisii Region, Southwest Kenya. World Applied Sciences Journal, 21(8), 1128–1135.

Ningdyah, A. W., Alimuddin, A. H., & Jayuska, A. (2015). Uji toksisitas dengan metode BSLT ( Brine Shrimp Lethality Test ) terhadap hasil fraksinasi ekstrak kulit buah tampoi. Jurnal Kimia Katulistiwa, 4(1), 75–83.

Nkobole, N., Houghton, P. J., Hussein, A., & Lall, N. (2011). Antidiabetic Activity of Terminalia sericea Constituents. Natural Product Communications, 6(11), 1585–1588.

Purwakusumah, E. D., Rafi, M., Syafitri, U. D., Nurcholis, W., Agung, M., & Adzkiya, Z. (2014). Identification and Authentication of Jahe Merah Using Combination of FTIR Spectrocopy and Chemometrics. AGRITECH, 34(1), 82–87.

Roanisca, O., Mahardika, R. G., & Sari, F. I. P. (2019). Total Phenolic and Antioxidant Capacity of Acetone Extract of Tristaniopsis meguensis Leaves. Stannum : Jurnal Sains Dan Terapan Kimia, 1(1), 10–13.

Roanisca, O., Mahardika, R. G., & Setiawan, Y. (2019). Tristaniopsis merguensis Griff. Extract as Inhibitor for Corrosion of Stainless Steel IOP Conference Series: Earth and Environmental Science, 353 012020.

Saraswaty, V. (2010). Alpha Glucosidase Inhibitory Activity From Syzigium sp. Teknologi Indonesia, 33(1), 33–37.

Sari, A. N., Kusdianti, & Diningrat, S. D. (2018). Analisis Gc-Ms Senyawa Bioaktif Pencegah Penyakit Degeneratif Dari Ekstrak Etanol Kulit Buah Jamblang (Syzygium cumini). Elkawnie: Journal of Islamic Science and Technology, 4(2), 1–14.

Sun, W., Sang, Y., Zhang, B., Yu, X., Xu, Q., Xiu, Z., & Dong, Y. (2017). Synergistic effects of acarbose and an Oroxylum indicum seed extract in streptozotocin and high-fat-diet induced prediabetic mice. Biomedicine et Pharmacotherapy, 87, 160–170.

Swantara, I. M. D., Rita, W. S., Made, I., & Suardhyana, A. (2016). Toksisitas senyawa flavonoid dari ekstrak etanol daun dewandaru (Eugenia uniflora Linn.) Sebagai skrining awal antikanker. Jurnal Kimia, 10(2), 181–189.

Verotta, L., Agli, M. D., Giolito, A., Guerrini, M., Cabalion, P., & Bosisio, E. (2001). In Vitro Antiplasmodial Activity of Extracts of Tristaniopsis Species and Identification of the Active Constituents : Ellagic Acid and 3 , 4 , 5-Trimethoxyphenyl- ( 6 ′ - O -galloyl ) - O - - D -glucopyranoside. Journal of Natural ProductNatural Product, 64(5), 603–607.



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P-ISSN : 2460-8912
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Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.

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