Anticancer Activity of N-Hexane Extract from Sphagneticola trilobata (L.) J.F Pruski Against MCF-7 Breast Cancer Cell
Abstract
Abstract : Sphagneticola trilobata (L.) J.F. Pruski is one of the perennial herbs that is widely used by the national and international community to treat various diseases including cancer. The objective of this study was to assessment the anticancer activity of n-hexane extract of S. trilobata leaves for inhibiting the growth of MCF-7 breast cancer cells in vitro by MTT (microculture tetrazolium salt) method. The n-hexane extract of sernai leaves was obtained from the maceration process of samples that were collected from the Langsa city, Aceh. The cytotoxicity test was carried out by incubating MCF-7 cells which had been exposed to several series of sample levels, viz. 1000; 500; 100; 50; 25; 10; 5 and 1 µg/mL. LC50 values are calculated using probit analysis. The results revealed that the n-hexane extract of S. trilobata leaves was cytotoxic against breast cancer cells (MCF-7) with an LC50 value of 0.037 μg /mL.
Abstrak : Sphagneticola trilobata (L.) J.F. Pruski merupakan salah satu tanaman herbal yang digunakan secara luas oleh masyarakat nasional dan internasional untuk mengobati berbagai penyakit termasuk kanker. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker ekstrak n-heksana daun S. trilobata dalam menghambat pertumbuhan sel kanker payudara MCF-7 secara in vitro dengan metode MTT (microculture tetrazolium salt). Ekstrak n-heksana daun sernai diperoleh dari proses maserasi sampel yang dikoleksi dari kota Langsa, Aceh. Uji sitotoksisitas dilakukan dengan menginkubasi sel MCF-7 yang telah dipaparkan beberapa seri kadar sampel yaitu 1000, 500, 100, 50, 25, 10, 5 dan 1 µg/mL. Nilai LC50 dihitung dengan menggunakan analisa probit. Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun S. trilobata bersifat sitotoksik terhadap sel kanker payudara (MCF-7) dengan harga LC50 sebesar 0,037 µg/mL.
Keywords
Full Text:
PDFReferences
Abate, G., Mshana, R. N., & Miörner, H. (1998). Evaluation of a colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. International Journal Tuberculosis Lung Dis, 2(12):1011–1016.
Ahmed, B.A., Idris1, S.N., Taha, R.M., Mustafa, M.M., & Marika, F. M. (2019). Phytochemical, pharmacological and tissue culture applications of Wedelia spp. – A review. Agricultural science and technology, 11(2), 123 – 132. http://dx.doi.org/10.15547/ast.2019.02.020
Ala, A.A., Olotu, B.B., & Ohia, C.M.D. (2018). Assessment of cytotoxicity of leaf extracts of Andrographis paniculata and Aspilia africana on murine cells in vitro. Arch Basic Appl Med, 6 (1): 61 – 65.
Antoney J, Britto A.J.D., Abida, P., & Raj, L.S. (2016). In-vitro cytotoxicity studies on methanolic leaf extract of Embelia ribes burm f - an important traditional medicinal plant of Kerala. Advances in Cytology and Pathology, 1(1), 6-8. https://doi.org/10.15406/acp.2016.01.00002
Arisanty, D. (2013). In vitro cytotoxic study and detectio of apoptosis on breast cancer cell lines MDA-MB 231 after exposed to Azadirachta Indica A. Juss (neem) extract. Jurnal Kesehatan Andalas, 2(2), 80 – 84. https://doi.org/10.25077/jka.v2i2.125
Balekar, N., Nakpheng, T., & Srichana, T. (2014). Wedelia trilobata L: a phytochemical and pharmacological review. Chiang Mai Journal Science, 41 (3): 590 – 605. http://dx.doi.org/10.22270/jddt.v9i4-s.3578.
Chethan, J., Kumara, K.K.S., Niranjana, S.R., & Prakash, H.S. (2012). Evaluation of antioxidant and antibacterial activities of methanolic flower extract of Wedelia trilobata (L.) Hitch. African Journal of Biotechnology, 11(41), 9829 – 9834. https://doi.org/10.5897/AJB11.3729.
Garbi, M.I., Osman, E.E., & Kabbashi, A.S. (2018). Anticancer activity of Bauhinia rufescens (Lam) leaf extracts on MCF-7 human breast cancer cells. Journal of Medicinal, 3(5), 103 – 106. https://doi.org/10.22271/plants.
Gowry, J., Sahayaraj, A., & Dharmalingam. (2014). Phytochemical screening and antimicrobial activity of different crude extracts of Wedelia biflora. Golden Research Thoughts, 4(6), 1- 6.
Husain, N., & Kumar, A. (2017). Characterization of antioxidant property of root extract of Sphagneticola trilobata in recovery of oxidative stress. Indian Journal Science Research, 12(12), 116 – 120. https://doi.org/10.13140/rg.2.2.13388.46724.
Kour, A. (2014). Review article: Plants exhibiting potential for cancer treatment. Int. J.Pharm. Sci. Rev.Res. 27(2), 23 – 53.
Mahfur. (2016).Uji sitotoksisitas fraksi etil asetat ekstrak etanol akar pasak bumi (Eurycoma longifolia jack) terhadap sel kanker T47D dengan metode 3-(4,5 dimetiltiazol-2-il)- 2,5 difenil tetrazolium bromide (MTT). Pena Jurnal Ilmu Pengetahuan dan Teknologi, 30(2), 57 – 63. http://dx.doi.org/10.31941/jurnalpena.v30i2.497.
Manjamalai, A. and Grace, B. (2013). Chemotherapeutic effect of essential oil of Wedelia chinensis (Osbeck) on inducing apoptosis, suppressing angiogenesis and lung metastasis in C57BL/6 mice model. Cancer science and therapy journal. 5(7), 271-281. DOI.10.4172/1948-5956.1000216.
Mardina, V., Al Fajar, B., & Fitriani. (2019). Potensi Phytomedicine Ekstrak Sphagneticola trilobata (L.) J.F Pruski Sebagai Agen Kemopreventif pada Kasus Kanker Payudara Mencit Terinduksi 7,12 Dimetilbenz(α)Antrasena. Jurnal Kedokteran Yarsi, 27(2), 52 – 61.https://doi.org/10.33476/jky.v27i2.
Mardina, V., Halimatussakdiah, Harmawan, T., Ilyas, S., Tanjung, M., Aulya, W., & Nasution, A. (2019). Preliminary phytochemical screening of different solvent extract of flower and whole plant of Wedelia biflora. 3rd Nomenmsen International Conference on Technology and Engineering, 25-26 July 2019, Medan-Indonesia.
Mishra, G., Singh, P., Garg, V.K., Parvez, N., Yadav, S., Hwisa, N., Molvi, K.I., Alsharif, S.M., Awev, B.Z., & Khosa, R.L. (2011). Phytochemical screening and anticonvulsant activity of Wedelia chinensis. International journal of pharmaceutical sciences and research, 2(1), 39 – 43. http://dx.doi.org/10.13040/IJPSR.0975-8232.2(1).25-29.
Rahardhian, M.R.R., & Utami, D. (2018). Uji sitotoksik dan antiproliferasi ekstrak eter daun (Andredera cordifolia (Tenore) Steen.) terhadap sel HeLa. Media Farmasi Indonesia, 13(1), 1284 – 1292.
Rahmawati, E., Sukardinam, & Muti, A.F. (2013). Aktivitas antikanker ekstrak n-heksana dan ekstrak metanol herba pacar air (Impatiens balsamina Linn) terhadap sel kenker payudara T47D. Media Farmasi, 10(2), 47 – 55. http://dx.doi.org/10.12928/mf.v10i2.1172.
Richard, T.S., Kamdje, A.H.N., & Mukhtar, F. (2015). Medicinal plants in breast cancer therapy. Journal of diseases and medicinal plants, 1(1), 19 – 23. DOI: 10.11648/j.jdmp.20150101.13.
Senthilraja, P., & Kathiresan, K. (2015). In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF7 cell lines study of marine yeast. Journal of applied pharmaceutical science, 5(3): 080 – 084. http://dx.doi.org/10.7324/JAPS.2015.50313.
Shankar, R. & Thomas, T. (2014). Antibacterial activity of flower heads of Wedelia trilobata (L.) A.S. Hitchc. Journal of Biological and Scientific Opinion, 2(6), 409 – 412. https://doi.org/10.7897/2321-6328.02692.
Taddei, A., & Rosas-Romero, A.J. (1999). Antimicrobial activity of Wedelia trilobata crude extracts. Phytomedicine, 6(2):133 – 134. https://doi.org/10.1016/S0944-7113(99)80048-8.
Thomy, Z., & Ginting, B. (2011). Isolation and cytotoxic test of plant secondary metabolites from Sernai (Wedelia biflora L.). Prosiding seminar nasional biologi “Meningkatkan peran biologi dalam mewujudkan national achievement with global reach. USU-Press: 282- 289.
Tsai, C.H., Lin, F.M., Yang, Y.C., lee, M.T., Cha, T.L., Wu, G.J., Hsieh,
S.C., & Hsiao, P.W. (2009). Herbal extract of Wedeilia chinensis attenuates androgem receptor activity and orthotopic growth of prostate cancer in nude mice. Clinical Cancer Center, 15(17): 534-544. DOI: 10.1158/1078-0432.CCR-09-0298.
Venkatesh, U., Kollur, S.P., Javarashetty, C., Jayarama, S., & Murari, S.K. (2016). Methanolic Extract of Wedelia trilobata in Antiproliferation and Apoptotic Activity. Natural Products Chemistry & Research. 4(2), 1-5. https://doi.org/10.4172/2329-6836.1000210.
Verma, N., & Khosa, R.L. Chemistry and biology of genus Wedelia Jacq.: A review. Indian journal of natural products and resources, 62 (June 2015): 71 – 90.
Wu, J., Li, H., Wang X., Zhang X, Liu, W., Wang, Y., Zhang, Y., Pan, H., Wang, Q., & Han, Y. (2019). Effect of polysaccharide from Undaria pinnatifida on proliferation migration and apoptosis of breast cancer cell MCF-7. International journal of biological macromolecules, 121(2019): 734 – 742. https://doi.org/10.1016/j.ijbiomac.2018.10.086.
DOI: http://dx.doi.org/10.22373/ekw.v6i1.6183
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Vivi Mardina, Tisna Harmawan, Halimatussakdiah Halimatussakdiah, Syafruddin Ilyas, Masitta Tanjung
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
P-ISSN : 2460-8912
E-ISSN : 2460-8920
ELKAWNIE
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.
View full page view stats report click here