Isolation and Identification of Lactic Acid Bacteria on Boiler Chicken
Abstract
Abstract: The aims of this research were to isolate lactic acid bacteria from broiler chicken. The main material was a chicken boiler aged 30 days. The identification of LAB was carried out based on isolation and identification of morphological, phenotypic, and biochemical characteristics. Thirteen LAB isolates were obtained and 5 selected LAB isolates were identified based on growth levels, pH, and lactic acid levels. Five selected LAB isolates were identified as Lactobacillus genus which has Gram-positive, negative catalase, non-motility, hetero-fermentative, rod shapes, rod-colony shapes, and cream colony color.
Abstrak: Penelitian ini bertujuan untuk mengisolasi bakteri asam laktat dari ayam broiler. Bahan utamanya adalah ayam boiler berumur 30 hari. Identifikasi BAL dilakukan berdasarkan isolasi dan identifikasi karakteristik morfologi, fenotipik, dan biokimia. Tiga belas isolat BAL diperoleh dan 5 isolat BAL terpilih diidentifikasi berdasarkan tingkat pertumbuhan, pH, dan kadar asam laktat. Lima isolat BAL terpilih diidentifikasi sebagai genus Lactobacillus yang memiliki Gram-positif, katalase negatif, non-motilitas, hetero-fermentatif, bentuk batang, bentuk koloni batang, dan warna koloni krem.
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Alghannam, A. F., Gonzalez, J. T., & Betts, J. A. (2018). Restoration of muscle glycogen and functional capacity: Role of post-exercise carbohydrate and protein co-ingestion. Nutrients, 10(2). https://doi.org/10.3390/nu10020253
Ardhana, M. M., & Fleet, G. H. (2003). The microbial ecology of cocoa bean fermentations in Indonesia. International Journal of Food Microbiology, 86(1–2), 87–99. https://doi.org/10.1016/S0168-1605(03)00081-3
Arief, I. I., Jenie, B. S. L., Astawan, M., Fujiyama, K., & Witarto, A. B. (2015). Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian Journal of Animal Sciences, 9(1), 25–36. https://doi.org/10.3923/ajas.2015.25.36
Astuti. (2010). Isolasi, karakterisasi dan aplikasi bakteri asam laktat dari saluran pencernaan ikan yang berpotensi menurunkan kadar kolesterol. Universitas Gadjah Mada Yogyakarta.
Blajman, J., Gaziano, C., Zbrun, M. V., Soto, L., Astesana, D., Berisvil, A., Scharpen, A. R., Signorini, M., & Frizzo, L. (2015). In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens. Research in Veterinary Science, 101, 50–56. https://doi.org/10.1016/j.rvsc.2015.05.017
Cappuccino, J. G., & Sherman, N. (2014). Microbiology: A Laboratory Manual (10th Editi). Pearson Education, Inc. United States of America.
Chen, X., Zhao, X., Qian, Y., Li, J., Chen, L., Chen, J., Zhang, Y., & Suo, H. (2017). Screening and identification of lactic acid bacteria strains with high acid-producing from traditional fermented yak yogurt. BIO Web of Conferences, 8, 03002. https://doi.org/10.1051/bioconf/20170803002
El-Ghaish, S., Dalgalarrondo, M., Choiset, Y., Sitohy, M., Ivanova, I., Haertlé, T., & Chobert, J. M. (2010). Characterization of a new isolate of Lactobacillus fermentum IFO 3956 from Egyptian Ras cheese with proteolytic activity. European Food Research and Technology, 230(4), 635–643. https://doi.org/10.1007/s00217-009-1206-x
Gómez, N. C., Ramiro, J. M. P., Quecan, B. X. V., & de Melo Franco, B. D. G. (2016). Use of potential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157: H7 biofilms formation. Frontiers in Microbiology, 7(JUN), 1–15. https://doi.org/10.3389/fmicb.2016.00863
Hawk, P. (1976). Hawk physiological chemistry (B. L. O. (ed) (ed.); 14th editi). Tata MC. Graw Hill Pub. co. Ltd.
Hernández-Aquino, S., Miranda-Romero, L. A., Fujikawa, H., de Jesús Maldonado-Simán, E., & Alarcón-Zuñiga, B. (2019). Antibacterial activity of lactic acid bacteria to improve shelf life of raw meat. Biocontrol Science, 24(4), 185–192. https://doi.org/10.4265/bio.24.185
Hidayati, D. (2010). Pola Pertumbuhan Bakteri Asam Laktat Selama Fermentasi Susu Kedelai. Jurnal Teknologi Hasil Pertanian, 3(2), 72. https://doi.org/10.20961/jthp.v0i0.13630
Idoui, T. (2014). Probiotic properties of Lactobacillus strains isolated from gizzard of local poultry. Iranian Journal of Microbiology, 6(2), 120–126.
Ismail, Y. S., Yulvizar, C., & Mazhitov, B. (2018). Characterization of lactic acid bacteria from local cows milk kefir. IOP Conference Series: Earth and Environmental Science, 130(1). https://doi.org/10.1088/1755-1315/130/1/012019
Kuda, T., Kataoka, M., Nemoto, M., Kawahara, M., Takahashi, H., & Kimura, B. (2016). Isolation of lactic acid bacteria from plants of the coastal Satoumi regions for use as starter cultures in fermented milk and soymilk production. LWT - Food Science and Technology, 68, 202–207. https://doi.org/10.1016/j.lwt.2015.12.023
Liu, W., Bao, Q., Jirimutu, Qing, M., Siriguleng, Chen, X., Sun, T., Li, M., Zhang, J., Yu, J., Bilige, M., Sun, T., & Zhang, H. (2012). Isolation and identification of lactic acid bacteria from Tarag in Eastern Inner Mongolia of China by 16S rRNA sequences and DGGE analysis. Microbiological Research, 167(2), 110–115. https://doi.org/10.1016/j.micres.2011.05.001
Mokoena, M. P. (2017). Lactic acid bacteria and their bacteriocins: Classification, biosynthesis and applications against uropathogens: A mini-review. Molecules, 22(8). https://doi.org/10.3390/molecules22081255
Monika, Savitri, Kumar, V., Kumari, A., Angmo, K., & Bhalla, T. C. (2017). Isolation and characterization of lactic acid bacteria from traditional pickles of Himachal Pradesh, India. Journal of Food Science and Technology, 54(7), 1945–1952. https://doi.org/10.1007/s13197-017-2629-1
Mulaw, G., Sisay Tessema, T., Muleta, D., & Tesfaye, A. (2019). In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented ethiopian food products. International Journal of Microbiology, 2019. https://doi.org/10.1155/2019/7179514
Nur, Z., & Aslim, B. (2010). Assessment of Potential Probiotic and Starter Properties of Pediococcus spp . Isolated from Turkish-Type Fermented Sausages ( Sucuk ). 20(November 2009), 161–168. https://doi.org/10.4014/jmb.0904.04019
Nurhayati. (2008). Seleksi dan identifikasi bakteri asam laktat yang berproliferasi pada tembolok dan usus halus ayam kampung. Universitas Gadjah Mada, Yogyakarta.
Ray, B., & Bhunia, A. (2013). Fundamental Food Microbiology (Fifth Edit). CRC Press.
Ren, D., Zhu, J., Gong, S., Liu, H., & Yu, H. (2018). Antimicrobial Characteristics of Lactic Acid Bacteria Isolated from Homemade Fermented Foods. BioMed Research International, 2018. https://doi.org/10.1155/2018/5416725
Romadhon, Subagiyo, & Margino, S. (2012). Isolasi Dan Karakterisasi Bakteri Asam Laktat Dari Usus Udang Penghasil Bakteriosin Sebagai Agen Antibakteria Pada Produk-Produk Hasil Perikanan. Indonesian Journal of Fisheries Science and Technology, 8(1), 59–64. https://doi.org/10.14710/ijfst.8.1.59-64
Sakaridis, I., Soultos, N., Batzios, C., Ambrosiadis, I., & Koidis, P. (2014). Lactic Acid Bacteria Isolated from Chicken Carcasses with Inhibitory Activity against Salmonella spp. and Listeria monocytogenes. Czech Journal of Food Sciences, 32(1), 61–68. https://doi.org/10.17221/414/2012-cjfs
Sarika, A., Lipton, A., Aishwarya, M., & mol, R. R. (2018). Lactic Acid Bacteria from Marine Fish: Antimicrobial Resistance and Production of Bacteriocin Effective Against L. monocytogenes In Situ. Journal of Food: Microbiology, Safety & Hygiene, 03(01). https://doi.org/10.4172/2476-2059.1000128
Sirilun, S., Sivamaruthi, B. S., Kesika, P., Peerajan, S., & Chaiyasut, C. (2017). Lactic acid bacteria mediated fermented soybean as a potent nutraceutical candidate. Asian Pacific Journal of Tropical Biomedicine, 7(10), 930–936. https://doi.org/10.1016/j.apjtb.2017.09.007
Syah, S. P., Sumantri, C., Arief, I. I., & Taufik, E. (2017). Isolation and identification of indigenous lactic acid bacteria by sequencing the 16s rrna from dangke, a traditional cheese from enrekang, south sulawesi. Pakistan Journal of Nutrition, 16(5), 384–392. https://doi.org/10.3923/pjn.2017.384.392
Wright, A. ., & Axelsson, L. (2012). Lactic acid bacteria: an Introduction. In S. Lahtinen, A. C. Ouwehand, S. Salminen, & A. Wright (Eds.), Lctic Acid Bacteria (pp. 1–48). CRC Press.
Wu, R., Wang, W., Yu, D., Zhang, W., Li, Y., Sun, Z., Wu, J., Meng, H., & Zhang, H. (2009). Proteomics analysis of Lactobacillus casei Zhang, a new probiotic bacterium isolated from traditional home-made Koumiss in Inner Mongolia of China. Molecular and Cellular Proteomics, 8(10), 2321–2338. https://doi.org/10.1074/mcp.M800483-MCP200
Yunilas, Y., Warly, L., & Mirwandhono, E. (2014). Toward Probiotict Food Product from Meat Through Isolation and Identification Lactic Acid Bacteria As Probiotic Culture Stater. International Journal on Advanced Science, Engineering and Information Technology, 4(2), 117. https://doi.org/10.18517/ijaseit.4.2.381
DOI: http://dx.doi.org/10.22373/ekw.v6i2.7015
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Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.
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