Toxicity Test Using The Brine Shrimp Lethality Test (BSLT) Method on Extracts of Stem Bark, Stem Wood, and Leaves on Bayur (Pterospermum diversifolium B. Rob.)
Abstract
Abstract: Ethnobotanically, the leaves of P. diversifolium are used as a medicine for itching and the root bark is used as fish poison. The traditional use of natural materials should be followed by scientific studies. This study aims to determine the toxicity of the stem bark, stem wood, and leaf tissue extracts of P. diversifolium using the Brine Shrimp Lethality Test (BSLT) method. Extraction using maceration method with ethanol solvent for 3 x 24 hours. The macerate was filtered and the extract obtained was evaporated until a crude extract was obtained. The three ethanol extracts obtained were tested for toxicity and obtained LC50 values of stem bark = 4,753 ppm, stem wood = 97,723 ppm, and leaves = 27,797 ppm. All extracts were declared non-toxic because the LC50 value was more than 1000 ppm.
Abstrak: Secara etnobotani, daun P. diversifolium digunakan sebagai obat gatal serta kulit akarnya sebagai racun ikan. Penggunaan bahan alam secara tradisional seharusnya diikuti kajian ilmiah. Penelitian ini bertujuan untuk mengetahui toksisitas pada ekstrak jaringan kulit batang, kayu batang, dan daun P.diversifolium dengan metode Brine Shrimp Lethality Test (BSLT). Ekstraksi menggunakan metode maserasi dengan pelarut etanol selama 3 x 24 jam. Maserat disaring dan ekstrak yang peroleh dievaporasi sampai didapatkan ekstrak kental. Ketiga ekstrak etanol yang diperoleh diuji toksisitasnya dan diperoleh nilai LC50 kulit batang = 4.753 ppm, kayu batang = 97.723 ppm, dan daun = 27.797 ppm. Semua ekstrak dinyatakan tidak toksik karena nilai LC50 lebih dari 1.000 ppm.
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Al-Enazi, N. M. (2018). Phytochemical screening and biological activities of some species of alpinia and Convolvulus plants. International Journal of Pharmacology, 14(3), pp. 301–309.
Altamish, M., Khan, M., Baig, M.S., Pathak, B., Rani, V., Akhtar, J., Khan, A.A., Ahmad, S., & Krishnan, A. (2022). Therapeutic Potential of Medicinal Plants against Dengue Infection: A Mechanistic Viewpoint. ACS Omega, (7). pp. 24048-24065.
Andila, P.S., Warseno, T., Hendra Wibawa, P.A., I Tirta, I.G. (2021). Ethnobotanical and Phytochemical Study of Bayur (Pterospermum javanicum Jungh.) on Sasak Tribe around Mount Rinjani National Park, West Lombok as a Conservation Effort. Journal of Tropical Biodiversity and Biotechnology, 6(1), pp. 1-11.
Anthony, W.E., Wang B, & Sukhum, K. V. (2022). Acute and persistent effects of commonly used antibiotics on the gut microbiome and resistome in healthy adults. Cell Rep 2022; 39:110649.
Dali, N., Dali, S., Chairunnas, A., Amalia, H. A. M., & Puspitasari, S. A. A. (2022). Extraction of Chemical Components of Dengen Leaves (Dillenia serrata Thunb) by MAE Method and Activity Test as Antioxidant and Toxicity. Indo. J. Chem. Res., 10(2), pp. 74-82.
Daud, N. S., Arni, D. P., Idris, S. A., & Saehu, M. S. (2023). Uji Aktivitas Antibakteri Ekstrak Batang Meistera chinensis Terhadap Escherichia coli ATCC 35218. Warta Farmasi, 12(1), pp. 8-18.
Hamrun, N., Nabilah, T., Hasyim, R., Ruslin, M., Dammar, I., & Arianto, M. A. A. (2020). Toxicity test of bioactive red alga extract Eucheuma spinosum on shrimp Artemia salina leach. Systematic Reviews in Pharmacy, 11(5), 672-676.
Hidayat, S. (2017). The use by local communities of plants from Sesaot Protected Forest, West Nusa Tenggara, Indonesia. Biodiversitas Journal of Biological Diversity, 18(1), pp. 238-247.
Hidayathulla, S., Chandra, K. K., & Chandrashekar, K. R. (2011). Phytochemical evaluation and antibacterial activity of Pterospermum diversifolium Blume. Int. J. Pharm. Sci., 3(2), pp. 165-167.
Jabbar, A., Wahyuni, W., Yusuf, M. I., Helmia, W. O. N., & Sahidin, I. (2019). Acute Toxicity Test of Ethanol Extract of Wualae Fruit (Etlingera elatior (Jack) R. M. Smith) Against Histopathological Picture of Wistar Rat Heart Organs (Rattus norvegicus). Pharmauho: Journal of Pharmacy, Science, And Health, 5(2), pp. 9–13.
Kamble, Y. S., Patil, R. S., Sawant, S. S., Pawar, S. G., & Singh, E. A. (2010). Studies on plant used intraditional medicine in Bhilla Tribe of Maharashthra. Indian Journal of Traditional Knowledge, 9, pp. 591‐598.
Madhuri, V., Bhuimbar, Ashwini, N., Kulkarni, & Jai, S. G. (2011). Detoxification of Chlorpyriphos by Micrococcus luteus NCIM 2103, Bacillus subtilis NCIM 2010 and Pseudomonas aeruginosa NCIM 203. Research Journal of Environmental and Earth Sciences, 3(5), pp. 614-619.
Mani, D. N., Shukla, S., & Shukla, Y. N. (2020). Medicinal plant-derived therapeutics for treatment of neoplasms in modern and traditional systems of medicine. Journal of Medicinal and Aromatic Plant Sciences, 42(1-2), 1-29.
Marzuki, A., Rahman, L., & Mamada, S.S., (2019). Toxicity test of stem bark extract of Banyuru (Pterospermum celebicum miq.) using BSLT (brine shrimp lethality test) and cream irritation test. The 3rd International Conference On Science. Journal of Physics, 1341(7). pp. 1-9.
Mastura, Mauliza, & Hasby. (2022). Toxicity Test of Acehnese Plants Using the Brine Shrimp Lethality Test (BSLT) Method. EduChemia (Jurnal Kimia dan Pendidikan), 7(1), pp. 110-123.
Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., & McLaughlin, J.L. (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Journal of Medicinal Plant Research, 45, pp. 31-34.
Ministry of Environment and Forestry Republic of Indonesia, (2018). The State of Indonesia’s Forests 2018. Ministry of Environment and Forestry Republic of Indonesia. Jakarta.
Mubarak, Z., Chismirina, S., & Daulay, H. H. (2016). Aktivitas antibakteri ekstrak propolis alami dari sarang lebah terhadap pertumbuhan Enterococcus faecalis. Journal of Syiah Kuala Dentistry Society, 1(2), 175-186.
Ogata, Y. (Committee Members). (1995). Index of Medicinal Plants in Indonesia. PT Esai Indonesia. Edition II.
Oluwaniyi, O., & Obi, B. (2018). Phytochemical, Anti-nutritional and Toxicity Assessment of Moringa Oleifera Seeds, Stem Bark and Leaves Using Brine Shrimp (Artemia salina) Assay. Jordan Journal of Chemistry, 13(3), pp. 171–178.
Pecoraro, R., Scalisi, E. M., Messina, G., Fragalà, G., Ignoto, S., Salvaggio, A., ... & Brundo, M. V. (2021). Artemia salina: A microcrustacean to assess engineered nanoparticles toxicity. Microscopy Research and Technique, 84(3), 531-536.
Pitakpawasutthi, Y., Suwatronnakorn, M., Issaravanich, S., Palanuvej, C., & Nijsiri, R. (2021). In vitro cytotoxic, genotoxic, and antityrosinase activities of Clitoria macrophylla root. Journal of Advanced Pharmaceutical Technology & Research, 12(1), pp. 8–13.
Potu, V.V., Pendong, D.F., & Semuel, M.Y. (2021). Brine Shrimp Lethality Test (BSLT) Ekstrak Sarang Lebah Madu (Apis dorsata Binghami). Jurnal Pendidikan Biologi Undiksha, 8(3). pp. 138-144.
Rafiqah, Mastura, & Hasibuan, M.P. (2019). Uji Toksisitas Fraksi Etanol Tanaman Obat yang digunakan Masyarakat Menggunakan Metode Brine Shrimp Lethality Test. CHEMICA: Jurnal Pendidikan Kimia dan Ilmu Kimia, 2(1). pp. 14-20.
Rasyid, M.I., Yuliani, H., & Angraeni, L. (2020). Toxicity Test LC 50 of Pineung Nyen Teusalee Seeds ( Areca catechu ) Extract by Brine Shrimp Lethality Test (BSLT) Methode. IOP Conference Series Earth and Environmental Science, 515(1):012052.
Riaz, B., Zahoor, M. K., Zahoor, M. A., Majeed, H. N., Javed, I., Ahmad, A., Jabeen, F., Zulhussnain, M., & Sultana, K. (2018). Toxicity, Phytochemical Composition, and Enzyme Inhibitory Activities of Some Indigenous Weed Plant Extracts in Fruit Fly, Drosophila melanogaster. Evidence-Based Complementary and Alternative Medicine.
Saragih, G., Tamrin, Marpongahtun, Nasution, D. Y., & Abdillah, (2020). Phytochemical screening and toxicity of ethanolic extract of mangrove (Rhizophora mucronata) leaves from Langsa, Aceh Timur. Rasayan Journal of Chemistry, 13(1), pp. 476–480.
Whitehill, J.G.A., Bohlmann, J., & Krokene, P. (2023). Forest Insect—Plant Interactions. Forest Entomology and Pathology (7). pp. 169-204.
Winahyu, D.A., Fatmawati, N.K.A., & Husein, S. (2024). Uji Toksisitas Ekstrak Kulit Buah Durian (Artemia salina L.) dengan Metode Brine Shrimp Lethality Test (BSLT). Jurnal Ilmu Kedokteran dan Kesehatan, 11(1). pp. 164-165.
DOI: http://dx.doi.org/10.22373/ekw.v10i1.20380
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Elkawnie: Journal of Islamic Science and Technology in 2022. Published by Faculty of Science and Technology in cooperation with Center for Research and Community Service (LP2M), UIN Ar-Raniry Banda Aceh, Aceh, Indonesia.
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