PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA
DOI:
https://doi.org/10.22373/biotik.v5i1.2975Keywords:
DNA extraction method, Phenol-Chloroform method, KIT extractionAbstract
DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Aceh’s cow and Madura’s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolating DNA, and clear DNA ribbon. Meanwhile, KIT extraction method is easier, fast, and thick DNA produced.Downloads
References
Pereira, F, J. Carneiro, dan A. Amorim. 2008. Identification of Species with DNA-Based Technology: Current Progress and Challenges. Recent Patents on DNA & Gene Sequences, 2, 187-200.
Sari, EM, H. Jianlin, R.R. Noor, C. Sumantri, dan E.T. Margawati. 2016. Phylogenetic analysis of aceh cattle breed of Indonesia through mitochondrial D-loop region. Journal of genetic engineering and biotechnology 14:227-231.
Swaran, Y.C. 2014. Direct PCR in Forensic Science- An overview. Malaysian Journal of Forensic Sciences, 5(2):6-12.
Teranishi, H, dan K. Ouchi. 2014. Detection of bacteria, fungi, and viruses by a real-time PCR assay using universal primers and probes from blood in patients with febrile neutropenia. Kawasaki Medical Journal 40(1):1-11.
Rompre, A, P. Servais, J. Baudart, M.R. de-Roubin, dan P. Laurent. 2002. Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. Journal of Microbiological Methods, 49 : 31–54.
Rawat, S, G. Joshi, D. Annapurna, A. N. Arunkumar, dan N.N. Karaba. 2016. Standardization of DNA Extraction Method from Mature Dried Leaves and ISSR-PCR Conditions for Melia dubia Cav. A Fast Growing Multipurpose Tree Species. American Journal of Plant Sciences, 7, 437-445.
Fang, G, S. Hammar, dan R. Grumet. 1992. A Quick and Inexpensive Method for Removing Polysaccharides from Plant Genome DNA. BioTechniques, 13, 52-56.
Fatchiyah, E.L. Arumingtyas, S. Widyarti, dan S. Rahayu. 2011. Biologi Molekular Prinsip Dasar Analisis. Jakarta: Erlangga.
Sambrook, J, E.F. Fritsch, dan T. Miniatis. 1989. Molecular Clooning : A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
Buchanan R. W, Julie Kreyenbuhl, Julie M. Zito, and Anthony Lehman. 2002. The Schizophrenia PORT Pharmacological Treatment Recommendations: Conformance and Implications for Symptoms and Functional Outcome. Schizophrenia Bulletin, Vol. 28, No. 1, 200.
Byun, S.O, Q. Fang, H. Zhou, dan J.G.H. Hickford. 2009. An effective method for silver-staining DNA in large numbers of polyacrilamid gels. Anal Biochem, 385, 174-175.
Wulandhari, A. 2009. Optimalisasi Hasil Ekstraksi DNA dari Darah Segar Sapi Menggunakan High Salt Method dengan Perbandingan Darah dan Lysis Buffer pada Kecepatan Sentrifugasi Berbeda. Skripsi. Institut Pertanian Bogor. Bogor.
Budi, P.A, R.S. Purbowotiningrum, dan A.L.N. Aminin. 2011. Purifikasi DNA kromosom Geobacillus sp. dYtae14 menggunakan kolom silika dengan denaturan urea. Jurnal Sains dan Matematika, 19(4), 101-106.
Ebeling, W. 1974. Proteinase K From Trilirachium album Limber. Eur J Biochem, 47, 91-97.
Montgomery, G. W. dan J. A. Sise. 1990. Extraction DNA from sheep white blood cells. New Zealand Journal of Agricultural Research. Vol. 33 : 437-441.
Kado, C.I dan S.T. Liu. 1981. Rapid Procedure for Detection and Isolation of Large and Small Plasmids. Journal of Bacteriology. 145 (3), 1365-1373.
Renshaw et al. 2015. The room temperature preservation of filtered environmental DNA Samples and Assimilation Into a Phenol–Chloroform–Isoamyl Alcohol DNA Extraction. Journal Molecular Ecology Resources. Volume 15, Issue 1
January 2015 Pages 168–176.
Downloads
Published
Issue
Section
License
Authors who publish with BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.




