UJI TOKSISITAS DARI PENYALUT LAYAK MAKAN BERBASIS PATI SAGU (Metroxylon sagu)
DOI:
https://doi.org/10.22373/biotik.v5i1.2976Keywords:
Sago starch, Nutrient Agar, sago powder, Escherichia coli, ToxicityAbstract
A sago starch- based (Metroxylon sagu) edible coating with sago powder as a filler and glycerol as a plasticizer has been made.This research is expected to provide information about microbial sensitivity towards the sago starch- based edible coating which is eco-friendly and safe to be used. Requirements to determine food quality refers to the role of E.coli as a toxicity indicator to cause food damage. Toxicity testing of E. coli toward sago starch-based coating was conducted by using Kirby Bauer method. A standardized agar-disc paper diffusion procedure is one way to to determine antibiotic resistancy of bacteria. Bacterial resistancy toward antibiotics is determined by the diameter of inhibit zone formed. Microorganisms cultures requires media that contain nutrients and appropriate environment such as Nutrient Agar (NA). Based on E. coli toxicity testing conducted to the sago starch-based edible coating in 370C temperature and 48 hours of incubation, it is found that the edible coated has not anticeptic activity toward Escherichia coli because the inhibit zone is not formed on the sago starch- based coating. Therefore, the edible coating which has been made is healthy to be consumed.References
King and Mc. Nelly. 1982. dalam Whistler dan be Miller.
Wirjosentono, B. 2007. â€Penyediaan Plastisiser yang Layak Makan, Substantif, Terbarukan dan Ramah Lingkungan Menggunakan Tehnik Eterifikasi Katalisis Heterogen dan Esterifikasi dengan Gugus Alkiloil Jenuh dan Reaktif – Polimer Berbasis Bahan Baku Gliserol Residu Pabrik Biodieselâ€, Penelitian PPKS – USU– Departemen Pertanian RI, Medan, Indonesia.
Biogen. 2008. Amilase, http:// biogen. litbang. deptan. go. Id/terbitan/agrobio/agrobio-vol. tanggal akses 05 Mei 2011.
Fank C, Lu. 1991. Toksikologi Dasar; Organ sasaran dan Penilaian Resiko. Jakarta: UI, Press.
Soemirat Juli.1999. Toksikologi Lingkungan, Cetakan Pertama. Yogyakarta: Gajah Mada Universitas Press, hal 12-19.
Lay, Bibiana W. dan Hastono, Sugyo. 1992, Analisis Microba di Laboratorium, ed L, cetl Pt. Jakarta: Raja Grafindo Perdasa. Hal 75,129.
K. Brahmana,1998. Bakteri pada Makanan. USU. Pres
Hadiotomo, Ratna Siri. 1993. Mikrobiologi Dasar dalam Praktek,Teknik dan Prosedur Dasar Laboratorium. Jakarta: PT. Gramesia, Pustaka Utama.
Dwijoseputro. 1987. Dasar-dasar Mikrobiologi. Malang: Universitas Brawijaya, Djambatan.
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