UJI ANTAGONIS BAKTERI TERHADAP CENDAWAN PATOGEN PENYAKIT BLAS

Zuraidah Zuraidah, Qatrun Nida, Sri Wahyuni

Abstract


Damage to leaves, panicles, and stems of rice plants caused by fungal pathogens, Pyricularia grisea, which causes Blas. Control of this disease by the use of biological agents in the form of bacteria which is Bacillus cereus and Pseudomonas aeruginosa, and consortium. This research aims to determine the ability of these bacterial isolates to inhibit the growth of Pyricularia grisea. This research was arranged in a Completely Randomized Design (CRD) with 4 treatments and 3 replications. The working procedures in this research were sampling of pathogenic fungi, making Pyricularia grisea liquid inoculum, and testing bacterial antagonists for pathogenic fungi in vitro. Analysis of variance (ANAVA) was used, with Fcount = 802.66 and Ftable = 3.48 with a significant level of α = 0.05 (5%) proved that the isolates of the bacteria Bacillus cereus and Pseudomonas aeruginosa could play an antagonistic role towards the fungus Pyricularia grisea. The results of this research indicate that bacterial isolates were able to inhibit the growth of Pyricularia grisea by the formation of the largest average clear zone in the treatment of Bacillus cereus bacteria which was 9.57 mm. The clear zone in the fungicide is 9.53 mm, the consortium is 9.37 mm, and the Pseudomonas aeruginosa is 8.2 mm.

Keywords


Pyricularia grisea, bacterial isolates (Bacillus cereus and Pseudomonas aeruginosa), consortium, clear zone.

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References


Nurhayati, Umayah U, Juharto. 2012. Antagonism of Pseudomonas fluorescens Migule. Asal Tanah Rhizospheres Pisang, Cabe dan Jagung Terhadap Fusarium oxysporum f.sp. cubense (E.F.Sm) Sdny Penyebab penyakit Layu pada Pisang. Majalah Ilmiah Sriwijaya. Vol. 22 (15). h. 39-48.

Muharni, Hary W. 2011. Skrining Bakteri Kitinolitik Antagonis Terhadap Pertumbuhan Jamur Akar Putih (Rigidoporus lignosus) dari Rizosfir Tanaman Karet. Jurnal Penelitian Sains. Vol. 14 (1). h. 52-60.

Kharisma, Sheila D, Abdul C, Luqman QA. 2013. Ketahanan Beberapa Genotipe Padi Hibrida (Oryza sativa L.) terhadap Pyricularia oryzae Cav. Penyebab Penyakit Blas Daun Padi. Jurnal HPT Vol 1 (2). h. 20-29.

Ni P, Linda S, et.al. 2014. Identifikasi Jamur Endofit dari Biji Padi dan Uji Daya Hambatnya terhadap Pyricularia oryzae Cav. Secara in Vitro. E-Jurnal Agroekoteknologi Tropika., 3(2). h. 51-52.

Kustianto B, Kartowinoto S, Amir M, Harahap Z. 1982. Perbaikan Ketahanan Varietas Terhadap Penyakit Blas. Dalam Puslitbangtan (ed.), pp: 127-138, Penelitian Pemuliaan Padi, Badan Litbang Pertanian. Puslitbang Tanaman Pangan.

Bizandi D. Brandelli A. 2002. Characterization of Bacteriocin Producted by a Newly Isolated Bacillus sp. Strain A. Jurnal Appl Microbiol. Vol. 93. h. 512-519.

Dirmawati SR. 2005. Penurunan Itensitas Penyakit Pustule Bakteri Kedelai Melalui Strategi Cara Tanam Tumpangsari dan Penggunaan Agensia Hayati. Jurnal Agrijati. Vol. 1. h. 7-10.

Wahyudi AT, Astuti RI, Mubarik NR, Faulina SA. 2009. Detection and Cloning of a Gene Involved in Zwitermicin a Biosynthesis from Plant Growth Promoting Rhizobacterium Bacillus sp. CR64. Jurnal Biotechnol. Vol. 15. h. 9-14.

Santhini E, Pradeepa D, Angayarkanni T, Kamalakannan A. 2005. Identification of Biochemical markers for the Selection of Effective Strain of Pseudomonas flourescens against Phytium spp. Di dalam: Gnanamanickam SS, Balasubramaniam R, Anand N, editors. Procedings of The Asian Conference on Emerging Trends in Plant-Microbes Interactions. Chennai: Center for 295-Advanced Studies in Botany University of Madras. h. 295-303.

Mubarik NR, Mahagiani I, Putri AA, Santoso S, Rusmana I. 2010. Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Application as Biocontrol for Whitefly (Bemisia tabaci Genn.). Am Jurnal Agric Biol Sci. Vol. 5. h. 430-535.

Jaiganesh V, Eswaran A, Balabaskar P, Kannan C. 2007. Antagonistic Activity of Serratia marcescens Against Pyricularia oryzae. Not Bot Hort Agrobot Cluj. Vol. 35. h. 171-200.

Irma SBr, Sitepu, et.al, 2009. Uji Aktivitas Antimikroba Beberapa Ekstrak Bumbu Dapur terhadap Pertumbuhan Jamur Curvularia lunata (Wakk.) Boed. dan Aspergillus flavus Link., Jurnal E-Jurnal Agroekoteknologi Tropika, vol. 1(2), h. 109.

Nuniek H, et.al, 2009. Kitinase dan Mikroorganisme Kitinolitik: Isolasi. Karakterisasi dan Manfaatnya. Jurnal Indo. J. Chem, vol. 9 (1), h. 37-43.

Agus S, Prasetyo AE. 2013. Respons Curvularia lunata Penyebab Penyakit Bercak Daun Kelapa Sawit terhadap Berbagai Fungisida. Jurnal Fitopatologi Indonesia, Vol.9 (6). h. 170.

Yonathan AS, et.a.2015. Dampak Beberapa Fungisida Terhadap Pertumbuhan Koloni Jamur Metarhizium anisopliae (Metch) Sorokin di Laboratorium. Jurnal Online Agroekoteknologi. Vol. 3 (1). h. 151-154.

Wage K. 2009. Karakteristik dan Pertumbuhan Konsorsium Mikroba Lokal dalam Media Mengandung Minyak Bumi. Jurnal Teknik Lingkungan. Vol. 10(1). h. 115-127.

Yadi S, et. al. 2015. Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae. Jurnal Fitopatologi. Vol. 11 (2). h. 222-230.




DOI: http://dx.doi.org/10.22373/biotik.v8i1.6667

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